{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8(41)"],"submitter":["Van den Broeck L"],"pubmed_abstract":["Capturing cell-to-cell signals in a three-dimensional (3D) environment is key to studying cellular functions. A major challenge in the current culturing methods is the lack of accurately capturing multicellular 3D environments. In this study, we established a framework for 3D bioprinting plant cells to study cell viability, cell division, and cell identity. We established long-term cell viability for bioprinted Arabidopsis and soybean cells. To analyze the generated large image datasets, we developed a high-throughput image analysis pipeline. Furthermore, we showed the cell cycle reentry of bioprinted cells for which the timing coincides with the induction of core cell cycle genes and regeneration-related genes, ultimately leading to microcallus formation. Last, the identity of bioprinted "],"journal":["Science advances"],"pagination":["eabp9906"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9565790"],"repository":["biostudies-literature"],"pubmed_title":["Establishing a reproducible approach to study cellular functions of plant cells with 3D bioprinting."],"pmcid":["PMC9565790"],"pubmed_authors":["Melvin C","Van den Broeck L","Tahir MA","Schwartz MF","Nguyen T","Muhammad A","Madison I","Peters R","Hunt A","Sozzani R","Krishnamoorthy S","Gobble M","Stuiver M","Horn T","Spurney RJ","Li B"],"additional_accession":[]},"is_claimable":false,"name":"Establishing a reproducible approach to study cellular functions of plant cells with 3D bioprinting.","description":"Capturing cell-to-cell signals in a three-dimensional (3D) environment is key to studying cellular functions. A major challenge in the current culturing methods is the lack of accurately capturing multicellular 3D environments. In this study, we established a framework for 3D bioprinting plant cells to study cell viability, cell division, and cell identity. We established long-term cell viability for bioprinted Arabidopsis and soybean cells. To analyze the generated large image datasets, we developed a high-throughput image analysis pipeline. Furthermore, we showed the cell cycle reentry of bioprinted cells for which the timing coincides with the induction of core cell cycle genes and regeneration-related genes, ultimately leading to microcallus formation. Last, the identity of bioprinted ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2026-05-28T00:46:56.479Z","creation":"2024-10-17T22:31:51.096Z"},"accession":"S-EPMC9565790","cross_references":{"pubmed":["36240264"],"doi":["10.1126/sciadv.abp9906"]}}