biostudies-literatureUnknown8(41)Van den Broeck LCapturing cell-to-cell signals in a three-dimensional (3D) environment is key to studying cellular functions. A major challenge in the current culturing methods is the lack of accurately capturing multicellular 3D environments. In this study, we established a framework for 3D bioprinting plant cells to study cell viability, cell division, and cell identity. We established long-term cell viability for bioprinted Arabidopsis and soybean cells. To analyze the generated large image datasets, we developed a high-throughput image analysis pipeline. Furthermore, we showed the cell cycle reentry of bioprinted cells for which the timing coincides with the induction of core cell cycle genes and regeneration-related genes, ultimately leading to microcallus formation. Last, the identity of bioprinted Science advanceseabp9906https://www.ebi.ac.uk/biostudies/studies/S-EPMC9565790biostudies-literatureEstablishing a reproducible approach to study cellular functions of plant cells with 3D bioprinting.PMC9565790Melvin CVan den Broeck LTahir MASchwartz MFNguyen TMuhammad AMadison IPeters RHunt ASozzani RKrishnamoorthy SGobble MStuiver MHorn TSpurney RJLi BfalseEstablishing a reproducible approach to study cellular functions of plant cells with 3D bioprinting.Capturing cell-to-cell signals in a three-dimensional (3D) environment is key to studying cellular functions. A major challenge in the current culturing methods is the lack of accurately capturing multicellular 3D environments. In this study, we established a framework for 3D bioprinting plant cells to study cell viability, cell division, and cell identity. We established long-term cell viability for bioprinted Arabidopsis and soybean cells. To analyze the generated large image datasets, we developed a high-throughput image analysis pipeline. Furthermore, we showed the cell cycle reentry of bioprinted cells for which the timing coincides with the induction of core cell cycle genes and regeneration-related genes, ultimately leading to microcallus formation. Last, the identity of bioprinted 2022-01-01T00:00:00Z2022 Oct2026-05-28T00:46:56.479Z2024-10-17T22:31:51.096ZS-EPMC95657903624026410.1126/sciadv.abp9906