{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ventura I"],"funding":["Generalitat Valenciana","Centro de Investigación Translacional “San Alberto Magno” of the Universidad Católica de Valencia “San Vicente Mártir”"],"pagination":["11399"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9570217"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(19)"],"pubmed_abstract":["The Polyribonucleotide nucleotidyltransferase 1 gene (<i>PNPT1</i>) encodes polynucleotide phosphorylase (PNPase), a 3'-5' exoribonuclease involved in mitochondrial RNA degradation and surveillance and RNA import into the mitochondrion. Here, we have characterized the <i>PNPT1</i> promoter by in silico analysis, luciferase reporter assays, electrophoretic mobility shift assays (EMSA), chromatin immunoprecipitation (ChIP), siRNA-based mRNA silencing and RT-qPCR. We show that the Specificity protein 1 (SP1) transcription factor and Nuclear transcription factor Y (NFY) bind the <i>PNPT1</i> promoter, and have a relevant role regulating the promoter activity, <i>PNPT1</i> expression, and mitochondrial activity. We also found in Kaplan-Meier survival curves that a high expression of either PNPa"],"journal":["International journal of molecular sciences"],"pubmed_title":["SP1 and NFY Regulate the Expression of <i>PNPT1</i>, a Gene Encoding a Mitochondrial Protein Involved in Cancer."],"pmcid":["PMC9570217"],"funding_grant_id":["UCV/2021-168-002","GV/2020/179"],"pubmed_authors":["Ventura I","Prieto-Ruiz JA","Gomez-Tatay L","Revert-Ros F","Hernandez-Andreu JM","Revert F"],"additional_accession":[]},"is_claimable":false,"name":"SP1 and NFY Regulate the Expression of <i>PNPT1</i>, a Gene Encoding a Mitochondrial Protein Involved in Cancer.","description":"The Polyribonucleotide nucleotidyltransferase 1 gene (<i>PNPT1</i>) encodes polynucleotide phosphorylase (PNPase), a 3'-5' exoribonuclease involved in mitochondrial RNA degradation and surveillance and RNA import into the mitochondrion. Here, we have characterized the <i>PNPT1</i> promoter by in silico analysis, luciferase reporter assays, electrophoretic mobility shift assays (EMSA), chromatin immunoprecipitation (ChIP), siRNA-based mRNA silencing and RT-qPCR. We show that the Specificity protein 1 (SP1) transcription factor and Nuclear transcription factor Y (NFY) bind the <i>PNPT1</i> promoter, and have a relevant role regulating the promoter activity, <i>PNPT1</i> expression, and mitochondrial activity. We also found in Kaplan-Meier survival curves that a high expression of either PNPa","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2026-04-08T11:56:21.881Z","creation":"2024-11-13T10:07:57.471Z"},"accession":"S-EPMC9570217","cross_references":{"pubmed":["36232701"],"doi":["10.3390/ijms231911399"]}}