biostudies-literatureVentura IGeneralitat ValencianaCentro de Investigación Translacional “San Alberto Magno” of the Universidad Católica de Valencia “San Vicente Mártir”11399https://www.ebi.ac.uk/biostudies/studies/S-EPMC9570217biostudies-literatureUnknown23(19)The Polyribonucleotide nucleotidyltransferase 1 gene (<i>PNPT1</i>) encodes polynucleotide phosphorylase (PNPase), a 3'-5' exoribonuclease involved in mitochondrial RNA degradation and surveillance and RNA import into the mitochondrion. Here, we have characterized the <i>PNPT1</i> promoter by in silico analysis, luciferase reporter assays, electrophoretic mobility shift assays (EMSA), chromatin immunoprecipitation (ChIP), siRNA-based mRNA silencing and RT-qPCR. We show that the Specificity protein 1 (SP1) transcription factor and Nuclear transcription factor Y (NFY) bind the <i>PNPT1</i> promoter, and have a relevant role regulating the promoter activity, <i>PNPT1</i> expression, and mitochondrial activity. We also found in Kaplan-Meier survival curves that a high expression of either PNPaInternational journal of molecular sciencesSP1 and NFY Regulate the Expression of <i>PNPT1</i>, a Gene Encoding a Mitochondrial Protein Involved in Cancer.PMC9570217UCV/2021-168-002GV/2020/179Ventura IPrieto-Ruiz JAGomez-Tatay LRevert-Ros FHernandez-Andreu JMRevert FfalseSP1 and NFY Regulate the Expression of <i>PNPT1</i>, a Gene Encoding a Mitochondrial Protein Involved in Cancer.The Polyribonucleotide nucleotidyltransferase 1 gene (<i>PNPT1</i>) encodes polynucleotide phosphorylase (PNPase), a 3'-5' exoribonuclease involved in mitochondrial RNA degradation and surveillance and RNA import into the mitochondrion. Here, we have characterized the <i>PNPT1</i> promoter by in silico analysis, luciferase reporter assays, electrophoretic mobility shift assays (EMSA), chromatin immunoprecipitation (ChIP), siRNA-based mRNA silencing and RT-qPCR. We show that the Specificity protein 1 (SP1) transcription factor and Nuclear transcription factor Y (NFY) bind the <i>PNPT1</i> promoter, and have a relevant role regulating the promoter activity, <i>PNPT1</i> expression, and mitochondrial activity. We also found in Kaplan-Meier survival curves that a high expression of either PNPa2022-01-01T00:00:00Z2022 Sep2026-04-08T11:56:21.881Z2024-11-13T10:07:57.471ZS-EPMC95702173623270110.3390/ijms231911399