biostudies-literatureCosta VDCSIC Grupos-908 I+DANIICAPCSIC IniciaciónComisión Sectorial de Investigación CientíficaAgencia Nacional de Investigación e InnovaciónPEDECIBA12047https://www.ebi.ac.uk/biostudies/studies/S-EPMC9570357biostudies-literatureUnknown23(19)Lung cancer is the first leading cause of cancer-related deaths in the world. Aberrant glycosylation in lung tumors leads to the expression of tumor-associated carbohydrate structures, such as the Tn antigen, consisting of <i>N</i>-acetyl-galactosamine (GalNAc) linked to a serine or threonine residue in proteins (α-GalNAc-O-Ser/Thr). The Tn antigen can be recognized by the Macrophage Galactose/GalNAc lectin (MGL), which mediates various immune regulatory and tolerogenic functions, mainly by reprogramming the maturation of function of dendritic cells (DCs). In this work, we generated two different Tn-expressing variants from the Lewis-type lung murine cancer cell line LL/2, which showed different alterations in the <i>O</i>-glycosylation pathways that influenced the interaction with mouse MInternational journal of molecular sciencesLung Tumor Cells with Different Tn Antigen Expression Present Distinctive Immunomodulatory Properties.PMC9570357FCE1-2017-1-136094Grupos-908 I+Dto Teresa Freire and Valeria da CostaCSIC-IniciaciónID114Rodriguez-Zraquia SAMarino KVLores PCosta VDCosta MRabinovich GAFreire TLandeira MFestari MFvan Vliet SJfalseLung Tumor Cells with Different Tn Antigen Expression Present Distinctive Immunomodulatory Properties.Lung cancer is the first leading cause of cancer-related deaths in the world. Aberrant glycosylation in lung tumors leads to the expression of tumor-associated carbohydrate structures, such as the Tn antigen, consisting of <i>N</i>-acetyl-galactosamine (GalNAc) linked to a serine or threonine residue in proteins (α-GalNAc-O-Ser/Thr). The Tn antigen can be recognized by the Macrophage Galactose/GalNAc lectin (MGL), which mediates various immune regulatory and tolerogenic functions, mainly by reprogramming the maturation of function of dendritic cells (DCs). In this work, we generated two different Tn-expressing variants from the Lewis-type lung murine cancer cell line LL/2, which showed different alterations in the <i>O</i>-glycosylation pathways that influenced the interaction with mouse M2022-01-01T00:00:00Z2022 Oct2025-04-04T09:29:57.596Z2024-11-06T04:34:13.733ZS-EPMC95703573623335810.3390/ijms231912047