{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang H"],"funding":["the Student Scientific research training program of Zhejiang Agriculture and Forestry University","Lingyan Project of Science and Technology Department of Zhejiang Province","the Natural Science Foundation of Zhejiang province","Ministry of Agriculture, and the National Key Research and Development Program of China","the National College Students Innovation and Entrepreneurship Training Program in 2019 and 2021","Natural Science Foundation of Zhejiang province","Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs, PR China/State Key Laboratory Breeding Base of Cultivation and Physiology for Tropical Crops/Danzhou Investigation and Experiment Station of Tropica","Talent starting funding from Zhejiang Agriculture and Forestry University","the National Natural Science Foundation of China"],"pagination":["2465"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9570929"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(19)"],"pubmed_abstract":["<i>Anoectochilus roxburghii</i> (Wall.) Lindl has been used in Chinese herbal medicine for treating various ailments. However, its wild resources are endangered, and artificial cultivation of the plant is limited by the low regeneration rate of conventional propagation methods. The lack of <i>A. roxburghii</i> resources is detrimental to the commercial production of the plant and kinsenoside, which is unique to <i>Anoectochilus</i> species. To develop highly efficient methods for <i>A. roxburghii</i> micropropagation and find alternative resources for kinsenoside production, we created an induction, proliferation, and regeneration of PLBs (IPR-PLB) protocol for <i>A. roxburghii</i>. We also analyzed the kinsenoside and flavonoid contents during the induction and proliferation of PLBs. The best media of IPR-PLB for PLB induction and proliferation (secondary PLB induction and proliferation), shoot formation, and rooting medium were Murashige and Skoog (MS) + 3 mg/L 6-benzylaminopurine (6-BA) + 0.5 mg/L naphthaleneacetic acid (NAA) + 0.8 mg/L zeatin (ZT) + 0.2 mg/L 2,4-dichlorophenoxyacetic acid (2, 4-D), MS + 3 mg/L 6-BA + 0.5 mg/L NAA, and MS + 0.5 mg/L NAA, respectively. On these optimized media, the PLB induction rate was 89 ± 2.08%, secondary PLB induction rate was 120 ± 5%, secondary PLB proliferation rate was 400 ± 10% and 350 ± 10 % in terms of the quantity and biomass at approximately 1 month, shoot induction rate was 10.5 shoots/PLB mass, and root induction rate was 98%. All plantlets survived after acclimation. Darkness or weak light were essential for PLB proliferation, and light was crucial for PLB differentiation on these optimized media. The kinsenoside contents of PLBs and secondary PLBs were 10.38 ± 0.08 and 12.30 ± 0.08 mg/g fresh weight (FW), respectively. Moreover, the peak kinsenoside content during the proliferation of secondary PLBs was 34.27 ± 0.79 mg/g FW, which was slightly lower than that of the whole plant (38.68 ± 3.12 mg/g FW). Two flavonoids exhibited tissue- or temporal-specific accumulation patterns, and astragalin accumulated exclusively during the first 2 weeks of cultivation. The IPR-PLB protocol for <i>A. roxburghii</i> may facilitate the efficient micropropagation of <i>A. roxburghii</i> plants. Furthermore, the PLBs are a good alternative resource for kinsenoside production."],"journal":["Plants (Basel, Switzerland)"],"pubmed_title":["Induction, Proliferation, Regeneration and Kinsenoside and Flavonoid Content Analysis of the <i>Anoectochilus roxburghii</i> (Wall.) Lindl Protocorm-like Body."],"pmcid":["PMC9570929"],"funding_grant_id":["Nos.2018YFD1000800 and 2019YFD1000300","Nos. 2021KX0196 and 2021KX019","Nos. 202110341043 and 201910341005","Grant Nos.31872105, 31972221, 32002048, 31801862, and 32172595","Grant No. RRI-KLOF202102","Grant No. LGG22H280001, Grant No. LY14H280008","Grant No. 2014FR062","Grant No.2022C02051","Nos. LY21C150002 and LQY19C150001"],"pubmed_authors":["Wei M","Tou L","Wang H","Chen X","Yan X","Shao Q","Fang L","Xu Z","Wu X"],"additional_accession":[]},"is_claimable":false,"name":"Induction, Proliferation, Regeneration and Kinsenoside and Flavonoid Content Analysis of the <i>Anoectochilus roxburghii</i> (Wall.) Lindl Protocorm-like Body.","description":"<i>Anoectochilus roxburghii</i> (Wall.) Lindl has been used in Chinese herbal medicine for treating various ailments. However, its wild resources are endangered, and artificial cultivation of the plant is limited by the low regeneration rate of conventional propagation methods. The lack of <i>A. roxburghii</i> resources is detrimental to the commercial production of the plant and kinsenoside, which is unique to <i>Anoectochilus</i> species. To develop highly efficient methods for <i>A. roxburghii</i> micropropagation and find alternative resources for kinsenoside production, we created an induction, proliferation, and regeneration of PLBs (IPR-PLB) protocol for <i>A. roxburghii</i>. We also analyzed the kinsenoside and flavonoid contents during the induction and proliferation of PLBs. The best media of IPR-PLB for PLB induction and proliferation (secondary PLB induction and proliferation), shoot formation, and rooting medium were Murashige and Skoog (MS) + 3 mg/L 6-benzylaminopurine (6-BA) + 0.5 mg/L naphthaleneacetic acid (NAA) + 0.8 mg/L zeatin (ZT) + 0.2 mg/L 2,4-dichlorophenoxyacetic acid (2, 4-D), MS + 3 mg/L 6-BA + 0.5 mg/L NAA, and MS + 0.5 mg/L NAA, respectively. On these optimized media, the PLB induction rate was 89 ± 2.08%, secondary PLB induction rate was 120 ± 5%, secondary PLB proliferation rate was 400 ± 10% and 350 ± 10 % in terms of the quantity and biomass at approximately 1 month, shoot induction rate was 10.5 shoots/PLB mass, and root induction rate was 98%. All plantlets survived after acclimation. Darkness or weak light were essential for PLB proliferation, and light was crucial for PLB differentiation on these optimized media. The kinsenoside contents of PLBs and secondary PLBs were 10.38 ± 0.08 and 12.30 ± 0.08 mg/g fresh weight (FW), respectively. Moreover, the peak kinsenoside content during the proliferation of secondary PLBs was 34.27 ± 0.79 mg/g FW, which was slightly lower than that of the whole plant (38.68 ± 3.12 mg/g FW). Two flavonoids exhibited tissue- or temporal-specific accumulation patterns, and astragalin accumulated exclusively during the first 2 weeks of cultivation. The IPR-PLB protocol for <i>A. roxburghii</i> may facilitate the efficient micropropagation of <i>A. roxburghii</i> plants. Furthermore, the PLBs are a good alternative resource for kinsenoside production.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2024-11-08T17:13:47.466Z","creation":"2024-11-08T17:13:47.466Z"},"accession":"S-EPMC9570929","cross_references":{"pubmed":["36235328"],"doi":["10.3390/plants11192465"]}}