{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Liu Z"],"funding":["Bing Liu","State Key Laboratory of NBC Protection for Civilian","Shasha Feng"],"pagination":["6977"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9573059"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(19)"],"pubmed_abstract":["A quantitative structure-activity relationship (QSAR) model for the structure and affinity of abrin aptamers was established. A higher affinity abrin aptamer based on the established QSAR model was screened by site-directed mutagenesis. The fluorescence quenching effect between magnetic microspheres and fluorescent molecules was studied for the first time. A new method for abrin detection based on the interaction between target molecules and fluorescently labeled aptamers on magnetic microspheres was developed, with the detection limit of 5 ng mL<sup>-1</sup>. This method can overcome the influence of complex environmental interferents in abrin detection and can meet the analysis requirements for simulated samples such as water, soil, and food."],"journal":["Materials (Basel, Switzerland)"],"pubmed_title":["A New Method for Abrin Detection Based on the Interaction between Target Molecules and Fluorescently Labeled Aptamers on Magnetic Microspheres."],"pmcid":["PMC9573059"],"funding_grant_id":["SKLNBC2020-07","SKLNBC2020-02"],"pubmed_authors":["Liu Z","Du B","Mu X","Tong Z","Liu B","Liu S","Feng S","Wang J","Wu Y","Xu J"],"additional_accession":[]},"is_claimable":false,"name":"A New Method for Abrin Detection Based on the Interaction between Target Molecules and Fluorescently Labeled Aptamers on Magnetic Microspheres.","description":"A quantitative structure-activity relationship (QSAR) model for the structure and affinity of abrin aptamers was established. A higher affinity abrin aptamer based on the established QSAR model was screened by site-directed mutagenesis. The fluorescence quenching effect between magnetic microspheres and fluorescent molecules was studied for the first time. A new method for abrin detection based on the interaction between target molecules and fluorescently labeled aptamers on magnetic microspheres was developed, with the detection limit of 5 ng mL<sup>-1</sup>. This method can overcome the influence of complex environmental interferents in abrin detection and can meet the analysis requirements for simulated samples such as water, soil, and food.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2026-04-08T11:50:25.915Z","creation":"2024-11-19T23:24:57.349Z"},"accession":"S-EPMC9573059","cross_references":{"pubmed":["36234322"],"doi":["10.3390/ma15196977"]}}