{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yan Q"],"funding":["Beijing Hospital Clinical Research 121 Project","Innovation project of Peking Union Medical College","Discipline Construction Project of Peking Union Medical College","Beijing Municipal Science and Technology Project","National Natural Science Foundation of China"],"pagination":["e1084"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9574492"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(10)"],"pubmed_abstract":["<h4>Background</h4>Identifying cellular and functional heterogeneity within aged prostate is critical for understanding the spatial distribution of prostate diseases.<h4>Methods</h4>Aged human prostate peripheral zone (PZ) and transitional zone (TZ) tissues were used for single-cell RNA-sequencing. Results were validated by immunofluorescence staining.<h4>Results</h4>We found that club/hillock epithelial cells, compared with other epithelial cells, had significantly higher NOTCH signaling activity and expressed higher levels of neuro-stems but lower androgen-related genes. These cells were primarily found in the TZ and provided a stem-like niche around the proximal prostate ducts. Significant heterogeneity was observed in the aged luminal population. A novel TFF3+ luminal subtype with elev"],"journal":["Clinical and translational medicine"],"pubmed_title":["Single-cell RNA-sequencing technology demonstrates the heterogeneity between aged prostate peripheral and transitional zone."],"pmcid":["PMC9574492"],"funding_grant_id":["201920202101","BJ-2018-090","2019-1002-69","81900700","Z201100005620007","82202900"],"pubmed_authors":["Wang M","Xia H","Yan Q","Dai C","Long X","Liu M","Zhang H","Diao T","Wang Y","Hou H"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell RNA-sequencing technology demonstrates the heterogeneity between aged prostate peripheral and transitional zone.","description":"<h4>Background</h4>Identifying cellular and functional heterogeneity within aged prostate is critical for understanding the spatial distribution of prostate diseases.<h4>Methods</h4>Aged human prostate peripheral zone (PZ) and transitional zone (TZ) tissues were used for single-cell RNA-sequencing. Results were validated by immunofluorescence staining.<h4>Results</h4>We found that club/hillock epithelial cells, compared with other epithelial cells, had significantly higher NOTCH signaling activity and expressed higher levels of neuro-stems but lower androgen-related genes. These cells were primarily found in the TZ and provided a stem-like niche around the proximal prostate ducts. Significant heterogeneity was observed in the aged luminal population. A novel TFF3+ luminal subtype with elev","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-27T03:35:17.501Z","creation":"2025-04-06T18:55:12.99Z"},"accession":"S-EPMC9574492","cross_references":{"pubmed":["36245324"],"doi":["10.1002/ctm2.1084"]}}