biostudies-literatureCorrales WANational Institute of Environmental Health SciencesIntramural NIH HHSFondo Nacional de Desarrollo Científico y Tecnológico2981-3001https://www.ebi.ac.uk/biostudies/studies/S-EPMC9575153biostudies-literatureUnknown12(16)We explored sex-biased effects of the primary stress glucocorticoid hormone corticosterone on the miRNA expression profile in the rat hippocampus. Adult adrenalectomized (ADX) female and male rats received a single corticosterone (10 mg/kg) or vehicle injection, and after 6 h, hippocampi were collected for miRNA, mRNA, and Western blot analyses. miRNA profiling microarrays showed a basal sex-biased miRNA profile in ADX rat hippocampi. Additionally, acute corticosterone administration triggered a sex-biased differential expression of miRNAs derived from genes located in several chromosomes and clusters on the X and 6 chromosomes. Putative promoter analysis unveiled that most corticosterone-responsive miRNA genes contained motifs for either direct or indirect glucocorticoid actions in both sACS chemical neuroscienceSex-Dependent Changes of miRNA Levels in the Hippocampus of Adrenalectomized Rats Following Acute Corticosterone Administration.PMC9575153ZIA ES090057Z01 ES0900571190899ZIC ES102445ZIC ES102546Silva JPAliaga ERojas PSMaracaja-Coutinho VAvalos AMAguayo FICidlowski JAVenegas-Zamora LFiedler JLRoman-Albasini LOakley RHParra CSOlave FACorrales WAfalseSex-Dependent Changes of miRNA Levels in the Hippocampus of Adrenalectomized Rats Following Acute Corticosterone Administration.We explored sex-biased effects of the primary stress glucocorticoid hormone corticosterone on the miRNA expression profile in the rat hippocampus. Adult adrenalectomized (ADX) female and male rats received a single corticosterone (10 mg/kg) or vehicle injection, and after 6 h, hippocampi were collected for miRNA, mRNA, and Western blot analyses. miRNA profiling microarrays showed a basal sex-biased miRNA profile in ADX rat hippocampi. Additionally, acute corticosterone administration triggered a sex-biased differential expression of miRNAs derived from genes located in several chromosomes and clusters on the X and 6 chromosomes. Putative promoter analysis unveiled that most corticosterone-responsive miRNA genes contained motifs for either direct or indirect glucocorticoid actions in both s2021-01-01T00:00:00Z2021 Aug2025-04-04T22:44:51.558Z2025-04-04T22:44:51.558ZS-EPMC95751533433916410.1021/acschemneuro.0c00762