biostudies-literatureLiu WInnovative Research Group Project of the National Natural Science Foundation of ChinaNational Medical Research Council Singapore44https://www.ebi.ac.uk/biostudies/studies/S-EPMC9575256biostudies-literatureUnknown11(1)<h4>Background</h4>Aberrant aggregation of α-synuclein (α-syn) is a key pathological feature of Parkinson's disease (PD), but the precise role of intestinal α-syn in the progression of PD is unclear. In a number of genetic Drosophila models of PD, α-syn was frequently ectopically expressed in the neural system to investigate the pathobiology.<h4>Method</h4>We investigated the potential role of intestinal α-syn in PD pathogenesis using a Drosophila model. Human α-syn was overexpressed in Drosophila guts, and life span, survival, immunofluorescence and climbing were evaluated. Immunofluorescence, Western blotting and reactive oxygen species (ROS) staining were performed to assess the effects of intestinal α-syn on intestinal dysplasia. High-throughput RNA and 16S rRNA gene sequencing, quantiTranslational neurodegenerationIntestine-derived α-synuclein initiates and aggravates pathogenesis of Parkinson's disease in Drosophila.PMC9575256STAR31501175OF LCG 000207Tan EKLim KLLiu WfalseIntestine-derived α-synuclein initiates and aggravates pathogenesis of Parkinson's disease in Drosophila.<h4>Background</h4>Aberrant aggregation of α-synuclein (α-syn) is a key pathological feature of Parkinson's disease (PD), but the precise role of intestinal α-syn in the progression of PD is unclear. In a number of genetic Drosophila models of PD, α-syn was frequently ectopically expressed in the neural system to investigate the pathobiology.<h4>Method</h4>We investigated the potential role of intestinal α-syn in PD pathogenesis using a Drosophila model. Human α-syn was overexpressed in Drosophila guts, and life span, survival, immunofluorescence and climbing were evaluated. Immunofluorescence, Western blotting and reactive oxygen species (ROS) staining were performed to assess the effects of intestinal α-syn on intestinal dysplasia. High-throughput RNA and 16S rRNA gene sequencing, quanti2022-01-01T00:00:00Z2022 Oct2026-05-27T19:09:21.961Z2025-02-19T03:22:59.307ZS-EPMC95752563625384410.1186/s40035-022-00318-w