biostudies-literatureKuwabara JTNaito FoundationAmerican Heart AssociationPaul G. Allen Frontiers GroupNHLBI NIH HHSNIMHD NIH HHSNCI NIH HHSNational Institutes of HealthNIH HHSNIGMS NIH HHSJapan Society for the Promotion of Sciencee69854https://www.ebi.ac.uk/biostudies/studies/S-EPMC9576271biostudies-literatureUnknown11Fibroblasts produce the majority of collagen in the heart and are thought to regulate extracellular matrix (ECM) turnover. Although fibrosis accompanies many cardiac pathologies and is generally deleterious, the role of fibroblasts in maintaining the basal ECM network and in fibrosis in vivo is poorly understood. We genetically ablated fibroblasts in mice to evaluate the impact on homeostasis of adult ECM and cardiac function after injury. Fibroblast-ablated mice demonstrated a substantive reduction in cardiac fibroblasts, but fibrillar collagen and the ECM proteome were not overtly altered when evaluated by quantitative mass spectrometry and N-terminomics. However, the distribution and quantity of collagen VI, microfibrillar collagen that forms an open network with the basement membrane, eLifeConsequences of PDGFRα⁺ fibroblast reduction in adult murine hearts.PMC9576271T32 HL115505R01 HL074257G12 MD007601P30 GM103341PRE29630019PRE834732GRNT33660474HL074257S10 OD023436HL115505S10 OD028515P30 CA071789Hara ALee AYZiolo MTBhutada SApte SSChen JKuwabara JTGojanovich GSHokutan KShettigar VHeckl JRDeAngelo LPJahansooz JRTacdol DKTallquist MDfalseConsequences of PDGFRα⁺ fibroblast reduction in adult murine hearts.Fibroblasts produce the majority of collagen in the heart and are thought to regulate extracellular matrix (ECM) turnover. Although fibrosis accompanies many cardiac pathologies and is generally deleterious, the role of fibroblasts in maintaining the basal ECM network and in fibrosis in vivo is poorly understood. We genetically ablated fibroblasts in mice to evaluate the impact on homeostasis of adult ECM and cardiac function after injury. Fibroblast-ablated mice demonstrated a substantive reduction in cardiac fibroblasts, but fibrillar collagen and the ECM proteome were not overtly altered when evaluated by quantitative mass spectrometry and N-terminomics. However, the distribution and quantity of collagen VI, microfibrillar collagen that forms an open network with the basement membrane, 2022-01-01T00:00:00Z2022 Sep2025-04-27T03:35:02.929Z2025-04-06T18:55:03.176ZS-EPMC95762713614905610.7554/eLife.69854