biostudies-literatureUnknown858(Pt 3)Islam MANorth South UniversityKTH Royal Institute of TechnologyWastewater based epidemiology (WBE) is an important tool to fight against COVID-19 as it provides insights into the health status of the targeted population from a small single house to a large municipality in a cost-effective, rapid, and non-invasive way. The implementation of wastewater based surveillance (WBS) could reduce the burden on the public health system, management of pandemics, help to make informed decisions, and protect public health. In this study, a house with COVID-19 patients was targeted for monitoring the prevalence of SARS-CoV-2 genetic markers in wastewater samples (WS) with clinical specimens (CS) for a period of 30 days. RT-qPCR technique was employed to target nonstructural (ORF1ab) and structural-nucleocapsid (N) protein genes of SARS-CoV-2, according to a validatThe Science of the total environment159350https://www.ebi.ac.uk/biostudies/studies/S-EPMC9576909biostudies-literatureA 30-day follow-up study on the prevalence of SARS-COV-2 genetic markers in wastewater from the residence of COVID-19 patient and comparison with clinical positivity.PMC9576909Hossain MSTasneem ASera FDidar-Ul-Alam MBhattacharya PAhmed TBahadur NMHasan MNMukharjee SKHaque MAIslam MTIslam MAJakariya MHossain ADhama KJahid IKAhmed FTiwari ARahman MAHossen FAmin RfalseA 30-day follow-up study on the prevalence of SARS-COV-2 genetic markers in wastewater from the residence of COVID-19 patient and comparison with clinical positivity.Wastewater based epidemiology (WBE) is an important tool to fight against COVID-19 as it provides insights into the health status of the targeted population from a small single house to a large municipality in a cost-effective, rapid, and non-invasive way. The implementation of wastewater based surveillance (WBS) could reduce the burden on the public health system, management of pandemics, help to make informed decisions, and protect public health. In this study, a house with COVID-19 patients was targeted for monitoring the prevalence of SARS-CoV-2 genetic markers in wastewater samples (WS) with clinical specimens (CS) for a period of 30 days. RT-qPCR technique was employed to target nonstructural (ORF1ab) and structural-nucleocapsid (N) protein genes of SARS-CoV-2, according to a validat2023-01-01T00:00:00Z2023 Feb2026-05-27T20:38:56.734Z2024-11-08T10:52:33.956ZS-EPMC95769093626562010.1016/j.scitotenv.2022.159350