{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhou L"],"funding":["Natural Science Foundation of China","European Regional Development Fund"],"pagination":["e202203045"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9577102"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["222(1)"],"pubmed_abstract":["At the trans-Golgi, complex traffic connections exist to the endolysosomal system additional to the main Golgi-to-plasma membrane secretory route. Here, we investigated three hits in a Drosophila screen displaying secretory cargo accumulation in autophagic vesicles: ESCRT-III component Vps20, SNARE-binding Rop, and lysosomal pump subunit VhaPPA1-1. We found that Vps20, Rop, and lysosomal markers localize near the trans-Golgi. Furthermore, we document that the vicinity of the trans-Golgi is the main cellular location for lysosomes and that early, late, and recycling endosomes associate as well with a trans-Golgi-associated degradative compartment where basal microautophagy of secretory cargo and other materials occurs. Disruption of this compartment causes cargo accumulation in our hits, in"],"journal":["The Journal of cell biology"],"pubmed_title":["Convergence of secretory, endosomal, and autophagic routes in trans-Golgi-associated lysosomes."],"pmcid":["PMC9577102"],"funding_grant_id":["31771600","31750410689","31701248"],"pubmed_authors":["Zhou L","Pastor-Pareja JC","Feng Z","Yang K","Liu M","Xue X"],"additional_accession":[]},"is_claimable":false,"name":"Convergence of secretory, endosomal, and autophagic routes in trans-Golgi-associated lysosomes.","description":"At the trans-Golgi, complex traffic connections exist to the endolysosomal system additional to the main Golgi-to-plasma membrane secretory route. Here, we investigated three hits in a Drosophila screen displaying secretory cargo accumulation in autophagic vesicles: ESCRT-III component Vps20, SNARE-binding Rop, and lysosomal pump subunit VhaPPA1-1. We found that Vps20, Rop, and lysosomal markers localize near the trans-Golgi. Furthermore, we document that the vicinity of the trans-Golgi is the main cellular location for lysosomes and that early, late, and recycling endosomes associate as well with a trans-Golgi-associated degradative compartment where basal microautophagy of secretory cargo and other materials occurs. Disruption of this compartment causes cargo accumulation in our hits, in","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2025-04-20T02:23:28.139Z","creation":"2025-04-20T02:23:28.139Z"},"accession":"S-EPMC9577102","cross_references":{"pubmed":["36239631"],"doi":["10.1083/jcb.202203045"]}}