biostudies-literatureZhou LNatural Science Foundation of ChinaEuropean Regional Development Funde202203045https://www.ebi.ac.uk/biostudies/studies/S-EPMC9577102biostudies-literatureUnknown222(1)At the trans-Golgi, complex traffic connections exist to the endolysosomal system additional to the main Golgi-to-plasma membrane secretory route. Here, we investigated three hits in a Drosophila screen displaying secretory cargo accumulation in autophagic vesicles: ESCRT-III component Vps20, SNARE-binding Rop, and lysosomal pump subunit VhaPPA1-1. We found that Vps20, Rop, and lysosomal markers localize near the trans-Golgi. Furthermore, we document that the vicinity of the trans-Golgi is the main cellular location for lysosomes and that early, late, and recycling endosomes associate as well with a trans-Golgi-associated degradative compartment where basal microautophagy of secretory cargo and other materials occurs. Disruption of this compartment causes cargo accumulation in our hits, inThe Journal of cell biologyConvergence of secretory, endosomal, and autophagic routes in trans-Golgi-associated lysosomes.PMC9577102317716003175041068931701248Zhou LPastor-Pareja JCFeng ZYang KLiu MXue XfalseConvergence of secretory, endosomal, and autophagic routes in trans-Golgi-associated lysosomes.At the trans-Golgi, complex traffic connections exist to the endolysosomal system additional to the main Golgi-to-plasma membrane secretory route. Here, we investigated three hits in a Drosophila screen displaying secretory cargo accumulation in autophagic vesicles: ESCRT-III component Vps20, SNARE-binding Rop, and lysosomal pump subunit VhaPPA1-1. We found that Vps20, Rop, and lysosomal markers localize near the trans-Golgi. Furthermore, we document that the vicinity of the trans-Golgi is the main cellular location for lysosomes and that early, late, and recycling endosomes associate as well with a trans-Golgi-associated degradative compartment where basal microautophagy of secretory cargo and other materials occurs. Disruption of this compartment causes cargo accumulation in our hits, in2023-01-01T00:00:00Z2023 Jan2025-04-20T02:23:28.139Z2025-04-20T02:23:28.139ZS-EPMC95771023623963110.1083/jcb.202203045