{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yang H"],"funding":["NIH Office of the Director","Division of Molecular and Cellular Biosciences","University of Wisconsin-Madison","National Institute of General Medical Sciences","NIGMS NIH HHS","NIH HHS"],"pagination":["11608-11619"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9577358"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["144(26)"],"pubmed_abstract":["NMR spectroscopy is a powerful tool to investigate molecular structure and dynamics. The poor sensitivity of this technique, however, limits its ability to tackle questions requiring dilute samples. Low-concentration photochemically induced dynamic nuclear polarization (LC-photo-CIDNP) is an optically enhanced NMR technology capable of addressing the above challenge by increasing the detection limit of aromatic amino acids in solution up to 1000-fold, either in isolation or within proteins. Here, we show that the absence of NMR-active nuclei close to a magnetically active site of interest (e.g., the structurally diagnostic <sup>1</sup>H<sup>α</sup>-<sup>13</sup>C<sup>α</sup> pair of amino acids) is expected to significantly increase LC-photo-CIDNP hyperpolarization. Then, we exploit the sp"],"journal":["Journal of the American Chemical Society"],"pubmed_title":["Selective Isotope Labeling and LC-Photo-CIDNP Enable NMR Spectroscopy at Low-Nanomolar Concentration."],"pmcid":["PMC9577358"],"funding_grant_id":["R01GM125995","MCB 2124672","S10OD012245","R01 GM125995","S10 OD012245","Bridge Funds"],"pubmed_authors":["Yang H","Dang HH","Cavagnero S","Li S","Guzman-Luna V","Thompson CM","Mickles CA","Sugisaki K"],"additional_accession":[]},"is_claimable":false,"name":"Selective Isotope Labeling and LC-Photo-CIDNP Enable NMR Spectroscopy at Low-Nanomolar Concentration.","description":"NMR spectroscopy is a powerful tool to investigate molecular structure and dynamics. The poor sensitivity of this technique, however, limits its ability to tackle questions requiring dilute samples. Low-concentration photochemically induced dynamic nuclear polarization (LC-photo-CIDNP) is an optically enhanced NMR technology capable of addressing the above challenge by increasing the detection limit of aromatic amino acids in solution up to 1000-fold, either in isolation or within proteins. Here, we show that the absence of NMR-active nuclei close to a magnetically active site of interest (e.g., the structurally diagnostic <sup>1</sup>H<sup>α</sup>-<sup>13</sup>C<sup>α</sup> pair of amino acids) is expected to significantly increase LC-photo-CIDNP hyperpolarization. Then, we exploit the sp","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jul","modification":"2025-04-05T12:21:01.339Z","creation":"2025-04-05T12:21:01.339Z"},"accession":"S-EPMC9577358","cross_references":{"pubmed":["35700317"],"doi":["10.1021/jacs.2c01809"]}}