{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12"],"submitter":["Hu D"],"pubmed_abstract":["Coronavirus disease 2019 (COVID-19) is an extremely contagious illness caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Early disease recognition of COVID-19 is crucial not only for prompt diagnosis and treatment of the patients, but also for effective public health surveillance and response. The reverse transcription-polymerase chain reaction (RT-PCR) is the most common method for the detection of SARS-CoV-2 viral mRNA and is regarded as the gold standard test for COVID-19. However, this test and those for antibodies (IgM and IgG) and antigens have certain limitations (e.g., by yielding false-negative and false-positive results). We have developed an RNA fluorescence <i>in situ</i> hybridization (FISH) method for high-sensitivity detection of SARS-CoV-2 mRNAs in HEK"],"journal":["Frontiers in cellular and infection microbiology"],"pagination":["960938"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9577401"],"repository":["biostudies-literature"],"pubmed_title":["Development of a high-sensitivity and short-duration fluorescence <i>in situ</i> hybridization method for viral mRNA detection in HEK 293T cells."],"pmcid":["PMC9577401"],"pubmed_authors":["Ma B","Hu D","Wang T","Uddin J","Greene WK"],"additional_accession":[]},"is_claimable":false,"name":"Development of a high-sensitivity and short-duration fluorescence <i>in situ</i> hybridization method for viral mRNA detection in HEK 293T cells.","description":"Coronavirus disease 2019 (COVID-19) is an extremely contagious illness caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Early disease recognition of COVID-19 is crucial not only for prompt diagnosis and treatment of the patients, but also for effective public health surveillance and response. The reverse transcription-polymerase chain reaction (RT-PCR) is the most common method for the detection of SARS-CoV-2 viral mRNA and is regarded as the gold standard test for COVID-19. However, this test and those for antibodies (IgM and IgG) and antigens have certain limitations (e.g., by yielding false-negative and false-positive results). We have developed an RNA fluorescence <i>in situ</i> hybridization (FISH) method for high-sensitivity detection of SARS-CoV-2 mRNAs in HEK","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-18T22:12:11.757Z","creation":"2025-04-07T10:00:20.956Z"},"accession":"S-EPMC9577401","cross_references":{"pubmed":["36268226"],"doi":["10.3389/fcimb.2022.960938"]}}