biostudies-literatureUnknown12Hu DCoronavirus disease 2019 (COVID-19) is an extremely contagious illness caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Early disease recognition of COVID-19 is crucial not only for prompt diagnosis and treatment of the patients, but also for effective public health surveillance and response. The reverse transcription-polymerase chain reaction (RT-PCR) is the most common method for the detection of SARS-CoV-2 viral mRNA and is regarded as the gold standard test for COVID-19. However, this test and those for antibodies (IgM and IgG) and antigens have certain limitations (e.g., by yielding false-negative and false-positive results). We have developed an RNA fluorescence <i>in situ</i> hybridization (FISH) method for high-sensitivity detection of SARS-CoV-2 mRNAs in HEKFrontiers in cellular and infection microbiology960938https://www.ebi.ac.uk/biostudies/studies/S-EPMC9577401biostudies-literatureDevelopment of a high-sensitivity and short-duration fluorescence <i>in situ</i> hybridization method for viral mRNA detection in HEK 293T cells.PMC9577401Ma BHu DWang TUddin JGreene WKfalseDevelopment of a high-sensitivity and short-duration fluorescence <i>in situ</i> hybridization method for viral mRNA detection in HEK 293T cells.Coronavirus disease 2019 (COVID-19) is an extremely contagious illness caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Early disease recognition of COVID-19 is crucial not only for prompt diagnosis and treatment of the patients, but also for effective public health surveillance and response. The reverse transcription-polymerase chain reaction (RT-PCR) is the most common method for the detection of SARS-CoV-2 viral mRNA and is regarded as the gold standard test for COVID-19. However, this test and those for antibodies (IgM and IgG) and antigens have certain limitations (e.g., by yielding false-negative and false-positive results). We have developed an RNA fluorescence <i>in situ</i> hybridization (FISH) method for high-sensitivity detection of SARS-CoV-2 mRNAs in HEK2022-01-01T00:00:00Z20222025-04-18T22:12:11.757Z2025-04-07T10:00:20.956ZS-EPMC95774013626822610.3389/fcimb.2022.960938