biostudies-literatureRoca-Lema DInstituto de Salud Carlos IIIAxencia Galega de Innovación“la Caixa” FoundationDeputación de A CoruñaMinisterio de Educación, Cultura y DeporteFundación Científica Asociación Española Contra el Cáncer17571https://www.ebi.ac.uk/biostudies/studies/S-EPMC9584894biostudies-literatureUnknown12(1)The E3 ubiquitin-ligases are important for cellular protein homeostasis and their deregulation is implicated in cancer. The E3 ubiquitin-ligase Hakai is involved in tumour progression and metastasis, through the regulation of the tumour suppressor E-cadherin. Hakai is overexpressed in colon cancer, however, the implication in colitis-associated cancer is unknown. Here, we investigated the potential role of Hakai in intestinal inflammation and cancer bowel disease. Several mouse models of colitis and associated cancer were used to analyse Hakai expression by immunohistochemistry. We also analysed Hakai expression in patients with inflamed colon biopsies from ulcerative colitis and Crohn's disease. By Hakai interactome analysis, it was identified Fatty Acid Synthase (FASN) as a novel Hakai-iScientific reportsRole of the E3 ubiquitin-ligase Hakai in intestinal inflammation and cancer bowel disease.PMC9584894PI21/00238PRDLC21591RODRIN607B2020/14LCF/TR/CC21/52490003FPU014/02837LCF/TR/CI19/52460016PI18/00121Concha ACerdan MERomay GQuiroga MKhare VGasche CDiaz-Diaz ARodriguez-Alonso AFigueroa ARoca-Lema DBarreiro-Alonso AfalseRole of the E3 ubiquitin-ligase Hakai in intestinal inflammation and cancer bowel disease.The E3 ubiquitin-ligases are important for cellular protein homeostasis and their deregulation is implicated in cancer. The E3 ubiquitin-ligase Hakai is involved in tumour progression and metastasis, through the regulation of the tumour suppressor E-cadherin. Hakai is overexpressed in colon cancer, however, the implication in colitis-associated cancer is unknown. Here, we investigated the potential role of Hakai in intestinal inflammation and cancer bowel disease. Several mouse models of colitis and associated cancer were used to analyse Hakai expression by immunohistochemistry. We also analysed Hakai expression in patients with inflamed colon biopsies from ulcerative colitis and Crohn's disease. By Hakai interactome analysis, it was identified Fatty Acid Synthase (FASN) as a novel Hakai-i2022-01-01T00:00:00Z2022 Oct2026-05-28T01:04:15.773Z2025-04-19T22:47:31.078ZS-EPMC95848943626642810.1038/s41598-022-22295-w