biostudies-literatureRamos-Pineda AMUniversidad de SalamancaSecretar?a de Estado de Investigaci?n, Desarrollo e Innovaci?nConsejer?a de Educaci?n, Junta de Castilla y Le?n13027-13035https://www.ebi.ac.uk/biostudies/studies/S-EPMC9585572biostudies-literatureUnknown70(41)Astringency is a highly complex sensation which involves multiple mechanisms occurring simultaneously, such as the interaction between flavan-3-ols and salivary proteins (SP). Moreover, astringency development can be affected by the presence of polysaccharides such as mannoproteins (MP). The aim of this work was to evaluate the molecular mechanisms whereby MP could modulate the astringency elicited by tannins, using a cell-based model of the oral epithelium (TR146 cells), and the effect of salivary proteins on these interactions. The binding of flavan-3-ols to oral cells was evaluated by DMACA assay, while the content of unbound flavan-3-ols after the interactions was assessed by means of HPLC-DAD-MS. Results obtained confirm the existence of cell-tannin interactions, that can be partiallyJournal of agricultural and food chemistryRole of Yeast Mannoproteins in the Interaction between Salivary Proteins and Flavan-3-ols in a Cell-Based Model of the Oral Epithelium.PMC9585572PIC2-2020-12CLU201804AGL2017-84793- C2-1-RSA0093P20Manjon EGarcia-Estevez IMacias RIREscribano-Bailon MTRamos-Pineda AMfalseRole of Yeast Mannoproteins in the Interaction between Salivary Proteins and Flavan-3-ols in a Cell-Based Model of the Oral Epithelium.Astringency is a highly complex sensation which involves multiple mechanisms occurring simultaneously, such as the interaction between flavan-3-ols and salivary proteins (SP). Moreover, astringency development can be affected by the presence of polysaccharides such as mannoproteins (MP). The aim of this work was to evaluate the molecular mechanisms whereby MP could modulate the astringency elicited by tannins, using a cell-based model of the oral epithelium (TR146 cells), and the effect of salivary proteins on these interactions. The binding of flavan-3-ols to oral cells was evaluated by DMACA assay, while the content of unbound flavan-3-ols after the interactions was assessed by means of HPLC-DAD-MS. Results obtained confirm the existence of cell-tannin interactions, that can be partially2022-01-01T00:00:00Z2022 Oct2025-04-26T00:55:33.336Z2025-04-06T09:54:03.959ZS-EPMC95855723564002410.1021/acs.jafc.1c08339