{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["5(12)"],"submitter":["Sconocchia G"],"pubmed_abstract":["The FcγRII (CD32) ligands are IgFc fragments and pentraxins. The existence of additional ligands is unknown. We engineered T cells with human chimeric receptors resulting from the fusion between CD32 extracellular portion and transmembrane CD8α linked to CD28/ζ chain intracellular moiety (CD32-CR). Transduced T cells recognized three breast cancer (BC) and one colon cancer cell line among 15 tested in the absence of targeting antibodies. Sensitive BC cell conjugation with CD32-CR T cells induced CD32 polarization and down-regulation, CD107a release, mutual elimination, and proinflammatory cytokine production unaffected by human IgGs but enhanced by cetuximab. CD32-CR T cells protected immunodeficient mice from subcutaneous growth of MDA-MB-468 BC cells. RNAseq analysis identified a 42 gene"],"journal":["Life science alliance"],"pagination":["e202201590"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9586128"],"repository":["biostudies-literature"],"pubmed_title":["Direct CD32 T-cell cytotoxicity: implications for breast cancer prognosis and treatment."],"pmcid":["PMC9586128"],"pubmed_authors":["Buccisano F","Chen K","Iezzi G","Venditti A","Maurillo L","Dou J","Toietta G","Cesarini V","Arriga R","Sconocchia G","Aureli A","Silvestris DA","Lanzilli G","De Paolis F","Spagnoli GC","Caratelli S","Del Principe MI","Rezvani K","Irno Consalvo M","Sconocchia T","Baldari S","Ottaviani A","Cenciarelli C"],"additional_accession":[]},"is_claimable":false,"name":"Direct CD32 T-cell cytotoxicity: implications for breast cancer prognosis and treatment.","description":"The FcγRII (CD32) ligands are IgFc fragments and pentraxins. The existence of additional ligands is unknown. We engineered T cells with human chimeric receptors resulting from the fusion between CD32 extracellular portion and transmembrane CD8α linked to CD28/ζ chain intracellular moiety (CD32-CR). Transduced T cells recognized three breast cancer (BC) and one colon cancer cell line among 15 tested in the absence of targeting antibodies. Sensitive BC cell conjugation with CD32-CR T cells induced CD32 polarization and down-regulation, CD107a release, mutual elimination, and proinflammatory cytokine production unaffected by human IgGs but enhanced by cetuximab. CD32-CR T cells protected immunodeficient mice from subcutaneous growth of MDA-MB-468 BC cells. RNAseq analysis identified a 42 gene","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2025-04-18T22:13:35.722Z","creation":"2025-02-18T23:59:31.34Z"},"accession":"S-EPMC9586128","cross_references":{"pubmed":["36241426"],"doi":["10.26508/lsa.202201590"]}}