biostudies-literatureUnknown5(12)Sconocchia GThe FcγRII (CD32) ligands are IgFc fragments and pentraxins. The existence of additional ligands is unknown. We engineered T cells with human chimeric receptors resulting from the fusion between CD32 extracellular portion and transmembrane CD8α linked to CD28/ζ chain intracellular moiety (CD32-CR). Transduced T cells recognized three breast cancer (BC) and one colon cancer cell line among 15 tested in the absence of targeting antibodies. Sensitive BC cell conjugation with CD32-CR T cells induced CD32 polarization and down-regulation, CD107a release, mutual elimination, and proinflammatory cytokine production unaffected by human IgGs but enhanced by cetuximab. CD32-CR T cells protected immunodeficient mice from subcutaneous growth of MDA-MB-468 BC cells. RNAseq analysis identified a 42 geneLife science alliancee202201590https://www.ebi.ac.uk/biostudies/studies/S-EPMC9586128biostudies-literatureDirect CD32 T-cell cytotoxicity: implications for breast cancer prognosis and treatment.PMC9586128Buccisano FChen KIezzi GVenditti AMaurillo LDou JToietta GCesarini VArriga RSconocchia GAureli ASilvestris DALanzilli GDe Paolis FSpagnoli GCCaratelli SDel Principe MIRezvani KIrno Consalvo MSconocchia TBaldari SOttaviani ACenciarelli CfalseDirect CD32 T-cell cytotoxicity: implications for breast cancer prognosis and treatment.The FcγRII (CD32) ligands are IgFc fragments and pentraxins. The existence of additional ligands is unknown. We engineered T cells with human chimeric receptors resulting from the fusion between CD32 extracellular portion and transmembrane CD8α linked to CD28/ζ chain intracellular moiety (CD32-CR). Transduced T cells recognized three breast cancer (BC) and one colon cancer cell line among 15 tested in the absence of targeting antibodies. Sensitive BC cell conjugation with CD32-CR T cells induced CD32 polarization and down-regulation, CD107a release, mutual elimination, and proinflammatory cytokine production unaffected by human IgGs but enhanced by cetuximab. CD32-CR T cells protected immunodeficient mice from subcutaneous growth of MDA-MB-468 BC cells. RNAseq analysis identified a 42 gene2022-01-01T00:00:00Z2022 Dec2025-04-18T22:13:35.722Z2025-02-18T23:59:31.34ZS-EPMC95861283624142610.26508/lsa.202201590