{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chaudhary P"],"funding":["NEI NIH HHS"],"pagination":["9"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9586137"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["63(11)"],"pubmed_abstract":["<h4>Purpose</h4>The purpose of this study was to test if optic nerve head (ONH) myelin basic protein (MBP), 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), glial fibrillary acidic protein (GFAP), and ionized calcium binding adaptor molecule 1 (Iba1) proteins are altered in non-human primate (NHP) early/moderate experimental glaucoma (EG).<h4>Methods</h4>Following paraformaldehyde perfusion, control and EG eye ONH tissues from four NHPs were paraffin embedded and serially (5 µm) vertically sectioned. Anti-MBP, CNPase, GFAP, Iba1, and nuclear dye-stained sections were imaged using sub-saturating light intensities. Whole-section images were segmented creating anatomically consistent laminar (L) and retrolaminar (RL) regions/sub-regions. EG versus control eye intensity/pixel-cluster den"],"journal":["Investigative ophthalmology & visual science"],"pubmed_title":["Optic Nerve Head Myelin-Related Protein, GFAP, and Iba1 Alterations in Non-Human Primates With Early to Moderate Experimental Glaucoma."],"pmcid":["PMC9586137"],"funding_grant_id":["R01 EY029087"],"pubmed_authors":["Chaudhary P","Yang H","Stowell C","Reynaud J","Williams I","Marsh-Armstrong N","Burgoyne CF","Williams G","Gardiner SK"],"additional_accession":[]},"is_claimable":false,"name":"Optic Nerve Head Myelin-Related Protein, GFAP, and Iba1 Alterations in Non-Human Primates With Early to Moderate Experimental Glaucoma.","description":"<h4>Purpose</h4>The purpose of this study was to test if optic nerve head (ONH) myelin basic protein (MBP), 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), glial fibrillary acidic protein (GFAP), and ionized calcium binding adaptor molecule 1 (Iba1) proteins are altered in non-human primate (NHP) early/moderate experimental glaucoma (EG).<h4>Methods</h4>Following paraformaldehyde perfusion, control and EG eye ONH tissues from four NHPs were paraffin embedded and serially (5 µm) vertically sectioned. Anti-MBP, CNPase, GFAP, Iba1, and nuclear dye-stained sections were imaged using sub-saturating light intensities. Whole-section images were segmented creating anatomically consistent laminar (L) and retrolaminar (RL) regions/sub-regions. EG versus control eye intensity/pixel-cluster den","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2026-07-14T15:08:16.799Z","creation":"2025-02-18T23:58:25.957Z"},"accession":"S-EPMC9586137","cross_references":{"pubmed":["36239974"],"doi":["10.1167/iovs.63.11.9"]}}