{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kuo YA"],"funding":["Texas 4000","Welch Foundation","National Institutes of Health","UT-Austin Catalyst Grant","NIGMS NIH HHS","NIH HHS","National Science Foundation"],"pagination":["e2204957"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9588665"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["34(41)"],"pubmed_abstract":["NanoCluster Beacons (NCBs) are multicolor silver nanocluster probes whose fluorescence can be activated or tuned by a proximal DNA strand called the activator. While a single-nucleotide difference in a pair of activators can lead to drastically different activation outcomes, termed polar opposite twins (POTs), it is difficult to discover new POT-NCBs using the conventional low-throughput characterization approaches. Here, a high-throughput selection method is reported that takes advantage of repurposed next-generation-sequencing chips to screen the activation fluorescence of ≈40 000 activator sequences. It is found that the nucleobases at positions 7-12 of the 18-nucleotide-long activator are critical to creating bright NCBs and positions 4-6 and 2-4 are hotspots to generate yellow-orange "],"journal":["Advanced materials (Deerfield Beach, Fla.)"],"pubmed_title":["Massively Parallel Selection of NanoCluster Beacons."],"pmcid":["PMC9588665"],"funding_grant_id":["F‐1155","F‐1808","F-1808","R21 GM129617","F-1155","CBET2029266","GM129617","CBET2041345","F-1833","F‐1833"],"pubmed_authors":["Wylie DC","Walker JN","Chen YA","Yeh HC","Nguyen TD","Kuo HC","Finkelstein IJ","Jung C","Rybarski JR","Brodbelt JS","Chen YI","Petty JT","Zhao OS","Hawkins JA","Kuo YA","Shields SWJ","Hong S"],"additional_accession":[]},"is_claimable":false,"name":"Massively Parallel Selection of NanoCluster Beacons.","description":"NanoCluster Beacons (NCBs) are multicolor silver nanocluster probes whose fluorescence can be activated or tuned by a proximal DNA strand called the activator. While a single-nucleotide difference in a pair of activators can lead to drastically different activation outcomes, termed polar opposite twins (POTs), it is difficult to discover new POT-NCBs using the conventional low-throughput characterization approaches. Here, a high-throughput selection method is reported that takes advantage of repurposed next-generation-sequencing chips to screen the activation fluorescence of ≈40 000 activator sequences. It is found that the nucleobases at positions 7-12 of the 18-nucleotide-long activator are critical to creating bright NCBs and positions 4-6 and 2-4 are hotspots to generate yellow-orange ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-25T21:32:10.021Z","creation":"2025-04-06T08:43:06.321Z"},"accession":"S-EPMC9588665","cross_references":{"pubmed":["35945159"],"doi":["10.1002/adma.202204957"]}}