{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Antony C"],"funding":["NICHD NIH HHS","NHLBI NIH HHS","NCI NIH HHS","NIGMS NIH HHS"],"pagination":["3826-3839.e9"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9588704"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["82(20)"],"pubmed_abstract":["Ribosomal RNAs (rRNAs) are the most abundant cellular RNAs, and their synthesis from rDNA repeats by RNA polymerase I accounts for the bulk of all transcription. Despite substantial variation in rRNA transcription rates across cell types, little is known about cell-type-specific factors that bind rDNA and regulate rRNA transcription to meet tissue-specific needs. Using hematopoiesis as a model system, we mapped about 2,200 ChIP-seq datasets for 250 transcription factors (TFs) and chromatin proteins to human and mouse rDNA and identified robust binding of multiple TF families to canonical TF motifs on rDNA. Using a 47S-FISH-Flow assay developed for nascent rRNA quantification, we demonstrated that targeted degradation of C/EBP alpha (CEBPA), a critical hematopoietic TF with conserved rDNA b"],"journal":["Molecular cell"],"pubmed_title":["Control of ribosomal RNA synthesis by hematopoietic transcription factors."],"pmcid":["PMC9588704"],"funding_grant_id":["R01 HD089245","R35 GM119735","R01 HL155144","U01 CA226187","R35 GM138035"],"pubmed_authors":["Moss T","Tan K","Wu-Corts DJ","Blum J","George SS","Wilusz JE","Pimkin M","Tremblay MG","Thorsheim CL","Antony C","Lv K","Paralkar VR","Gao L","Ai Y","Somers P","Ganley ARD"],"additional_accession":[]},"is_claimable":false,"name":"Control of ribosomal RNA synthesis by hematopoietic transcription factors.","description":"Ribosomal RNAs (rRNAs) are the most abundant cellular RNAs, and their synthesis from rDNA repeats by RNA polymerase I accounts for the bulk of all transcription. Despite substantial variation in rRNA transcription rates across cell types, little is known about cell-type-specific factors that bind rDNA and regulate rRNA transcription to meet tissue-specific needs. Using hematopoiesis as a model system, we mapped about 2,200 ChIP-seq datasets for 250 transcription factors (TFs) and chromatin proteins to human and mouse rDNA and identified robust binding of multiple TF families to canonical TF motifs on rDNA. Using a 47S-FISH-Flow assay developed for nascent rRNA quantification, we demonstrated that targeted degradation of C/EBP alpha (CEBPA), a critical hematopoietic TF with conserved rDNA b","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2026-05-28T01:39:36.15Z","creation":"2025-02-19T03:06:21.33Z"},"accession":"S-EPMC9588704","cross_references":{"pubmed":["36113481"],"doi":["10.1016/j.molcel.2022.08.027"]}}