biostudies-literatureAntony CNICHD NIH HHSNHLBI NIH HHSNCI NIH HHSNIGMS NIH HHS3826-3839.e9https://www.ebi.ac.uk/biostudies/studies/S-EPMC9588704biostudies-literatureUnknown82(20)Ribosomal RNAs (rRNAs) are the most abundant cellular RNAs, and their synthesis from rDNA repeats by RNA polymerase I accounts for the bulk of all transcription. Despite substantial variation in rRNA transcription rates across cell types, little is known about cell-type-specific factors that bind rDNA and regulate rRNA transcription to meet tissue-specific needs. Using hematopoiesis as a model system, we mapped about 2,200 ChIP-seq datasets for 250 transcription factors (TFs) and chromatin proteins to human and mouse rDNA and identified robust binding of multiple TF families to canonical TF motifs on rDNA. Using a 47S-FISH-Flow assay developed for nascent rRNA quantification, we demonstrated that targeted degradation of C/EBP alpha (CEBPA), a critical hematopoietic TF with conserved rDNA bMolecular cellControl of ribosomal RNA synthesis by hematopoietic transcription factors.PMC9588704R01 HD089245R35 GM119735R01 HL155144U01 CA226187R35 GM138035Moss TTan KWu-Corts DJBlum JGeorge SSWilusz JEPimkin MTremblay MGThorsheim CLAntony CLv KParalkar VRGao LAi YSomers PGanley ARDfalseControl of ribosomal RNA synthesis by hematopoietic transcription factors.Ribosomal RNAs (rRNAs) are the most abundant cellular RNAs, and their synthesis from rDNA repeats by RNA polymerase I accounts for the bulk of all transcription. Despite substantial variation in rRNA transcription rates across cell types, little is known about cell-type-specific factors that bind rDNA and regulate rRNA transcription to meet tissue-specific needs. Using hematopoiesis as a model system, we mapped about 2,200 ChIP-seq datasets for 250 transcription factors (TFs) and chromatin proteins to human and mouse rDNA and identified robust binding of multiple TF families to canonical TF motifs on rDNA. Using a 47S-FISH-Flow assay developed for nascent rRNA quantification, we demonstrated that targeted degradation of C/EBP alpha (CEBPA), a critical hematopoietic TF with conserved rDNA b2022-01-01T00:00:00Z2022 Oct2026-05-28T01:39:36.15Z2025-02-19T03:06:21.33ZS-EPMC95887043611348110.1016/j.molcel.2022.08.027