{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tanaka M"],"funding":["NHGRI NIH HHS","Research and Study Project of Tokai University General Research Organization","National Institute of General Medical Sciences","National Human Genome Research Institute","NIGMS NIH HHS","Tokai University School of Medicine Project Research"],"pagination":["228"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9594901"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(1)"],"pubmed_abstract":["CRISPR tools can generate knockout and knock-in animal models easily, but the models can contain off-target genomic lesions or random insertions of donor DNAs. Simpler methods to identify off-target lesions and random insertions, using tail or earpiece DNA, are unavailable. We develop CRISPR-KRISPR (CRISPR-Knock-ins and Random Inserts Searching PRotocol), a method to identify both off-target lesions and random insertions. CRISPR-KRISPR uses as little as 3.4 μg of genomic DNA; thus, it can be easily incorporated as an additional step to genotype founder animals for further breeding."],"journal":["Genome biology"],"pubmed_title":["CRISPR-KRISPR: a method to identify on-target and random insertion of donor DNAs and their characterization in knock-in mice."],"pmcid":["PMC9594901"],"funding_grant_id":["2016-2017","2016-2018","R21GM129559","R35HG010719"],"pubmed_authors":["Miura H","Hayashi H","Isaki S","Gurumurthy CB","Matsuzawa H","Wang T","Kawata H","Kitatani K","Yokoyama K","Ohtsuka M","Tanaka M"],"additional_accession":[]},"is_claimable":false,"name":"CRISPR-KRISPR: a method to identify on-target and random insertion of donor DNAs and their characterization in knock-in mice.","description":"CRISPR tools can generate knockout and knock-in animal models easily, but the models can contain off-target genomic lesions or random insertions of donor DNAs. Simpler methods to identify off-target lesions and random insertions, using tail or earpiece DNA, are unavailable. We develop CRISPR-KRISPR (CRISPR-Knock-ins and Random Inserts Searching PRotocol), a method to identify both off-target lesions and random insertions. CRISPR-KRISPR uses as little as 3.4 μg of genomic DNA; thus, it can be easily incorporated as an additional step to genotype founder animals for further breeding.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-18T11:42:26.14Z","creation":"2024-10-15T01:22:02.543Z"},"accession":"S-EPMC9594901","cross_references":{"pubmed":["36284311"],"doi":["10.1186/s13059-022-02779-8"]}}