biostudies-literatureUnknown13Li QNational Natural Science Foundation of ChinaAgriculture Research System of China<i>Medicago falcata</i> L. is an important legume forage grass with strong drought resistant, which could be utilized as an important gene pool in molecular breed of forage grass. In this study, <i>M. falcata</i> seedlings were treated with 400 mM mannitol to simulate drought stress, and the morphological and physiological changes were investigated, as well as the transcriptome changes of <i>M. falcata</i> seedlings at different treatment time points (0 h, 2 h, 6 h, 12 h, 24 h, 36 h and 48 h). Transcriptome analyses revealed four modules were closely related with drought response in <i>M. falcata</i> by WGCNA analysis, and four <i>ERF</i> transcription factor genes related with drought stress were identified (<i>MfERF053</i>, <i>MfERF9</i>, <i>MfERF034</i> and <i>MfRAP2.1</i>). Among them,Frontiers in plant science995754https://www.ebi.ac.uk/biostudies/studies/S-EPMC9594990biostudies-literatureTranscriptome and functional analyses reveal <i>ERF053</i> from <i>Medicago falcata as</i> key regulator in drought resistances.PMC9594990Jiang ZJiang WDu WLi QQiang ZWang YZhang BPang YSong JfalseTranscriptome and functional analyses reveal <i>ERF053</i> from <i>Medicago falcata as</i> key regulator in drought resistances.<i>Medicago falcata</i> L. is an important legume forage grass with strong drought resistant, which could be utilized as an important gene pool in molecular breed of forage grass. In this study, <i>M. falcata</i> seedlings were treated with 400 mM mannitol to simulate drought stress, and the morphological and physiological changes were investigated, as well as the transcriptome changes of <i>M. falcata</i> seedlings at different treatment time points (0 h, 2 h, 6 h, 12 h, 24 h, 36 h and 48 h). Transcriptome analyses revealed four modules were closely related with drought response in <i>M. falcata</i> by WGCNA analysis, and four <i>ERF</i> transcription factor genes related with drought stress were identified (<i>MfERF053</i>, <i>MfERF9</i>, <i>MfERF034</i> and <i>MfRAP2.1</i>). Among them,2022-01-01T00:00:00Z20222026-05-27T11:47:18.582Z2024-11-19T16:29:29.164ZS-EPMC95949903630439110.3389/fpls.2022.995754