{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(1)"],"submitter":["Piancone E"],"pubmed_abstract":["To date several studies address the important role of gut microbiome and its interplay with the human host in the health and disease status. However, the selection of a universal sampling matrix representative of the microbial biodiversity associated with the gastrointestinal (GI) tract, is still challenging. Here we present a study in which, through a deep metabarcoding analysis of the 16S rRNA gene, we compared two sampling matrices, feces (F) and colon washing feces (CWF), in order to evaluate their relative effectiveness and accuracy in representing the complexity of the human gut microbiome. A cohort of 30 volunteers was recruited and paired F and CWF samples were collected from each subject. Alpha diversity analysis confirmed a slightly higher biodiversity of CWF compared to F matche"],"journal":["Scientific reports"],"pagination":["17909"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9596478"],"repository":["biostudies-literature"],"pubmed_title":["Natural and after colon washing fecal samples: the two sides of the coin for investigating the human gut microbiome."],"pmcid":["PMC9596478"],"pubmed_authors":["Fosso B","Notario E","D'Erchia AM","Defazio G","Vergallo I","De Robertis M","Visci G","Filomena E","Manzari C","Piancone E","Pesole G","Bruno S","Maio D","Marzano M","Oranger A","Minelli M"],"additional_accession":[]},"is_claimable":false,"name":"Natural and after colon washing fecal samples: the two sides of the coin for investigating the human gut microbiome.","description":"To date several studies address the important role of gut microbiome and its interplay with the human host in the health and disease status. However, the selection of a universal sampling matrix representative of the microbial biodiversity associated with the gastrointestinal (GI) tract, is still challenging. Here we present a study in which, through a deep metabarcoding analysis of the 16S rRNA gene, we compared two sampling matrices, feces (F) and colon washing feces (CWF), in order to evaluate their relative effectiveness and accuracy in representing the complexity of the human gut microbiome. A cohort of 30 volunteers was recruited and paired F and CWF samples were collected from each subject. Alpha diversity analysis confirmed a slightly higher biodiversity of CWF compared to F matche","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2026-04-12T19:27:05.575Z","creation":"2026-04-07T13:17:56.919Z"},"accession":"S-EPMC9596478","cross_references":{"pubmed":["36284112"],"doi":["10.1038/s41598-022-20888-z"]}}