biostudies-literatureCarll APU.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS)NHLBI NIH HHSU.S. Department of Health & Human Services | NIH | National Heart, Lung, and Blood Institute (NHLBI)NIGMS NIH HHS6088https://www.ebi.ac.uk/biostudies/studies/S-EPMC9596490biostudies-literatureUnknown13(1)E-cigarette use has surged, but the long-term health effects remain unknown. E-cigarette aerosols containing nicotine and acrolein, a combustion and e-cigarette byproduct, may impair cardiac electrophysiology through autonomic imbalance. Here we show in mouse electrocardiograms that acute inhalation of e-cigarette aerosols disturbs cardiac conduction, in part through parasympathetic modulation. We demonstrate that, similar to acrolein or combustible cigarette smoke, aerosols from e-cigarette solvents (vegetable glycerin and propylene glycol) induce bradycardia, bradyarrhythmias, and elevations in heart rate variability during inhalation exposure, with inverse post-exposure effects. These effects are slighter with tobacco- or menthol-flavored aerosols containing nicotine, and in female miceNature communicationsE-cigarettes and their lone constituents induce cardiac arrhythmia and conduction defects in mice.PMC9596490P30 GM127607P30GM127607R01 HL163818R01HL147343R01HL163818R01 HL147343R01HL122676P50 HL120163U54HL120163R01 HL122676U54 HL120163Miles MDTheis WSBhatnagar ASalatini RShirk GARiggs DWNystoriak MAConklin DJArab CCarll APFulghum KLTalebi NfalseE-cigarettes and their lone constituents induce cardiac arrhythmia and conduction defects in mice.E-cigarette use has surged, but the long-term health effects remain unknown. E-cigarette aerosols containing nicotine and acrolein, a combustion and e-cigarette byproduct, may impair cardiac electrophysiology through autonomic imbalance. Here we show in mouse electrocardiograms that acute inhalation of e-cigarette aerosols disturbs cardiac conduction, in part through parasympathetic modulation. We demonstrate that, similar to acrolein or combustible cigarette smoke, aerosols from e-cigarette solvents (vegetable glycerin and propylene glycol) induce bradycardia, bradyarrhythmias, and elevations in heart rate variability during inhalation exposure, with inverse post-exposure effects. These effects are slighter with tobacco- or menthol-flavored aerosols containing nicotine, and in female mice2022-01-01T00:00:00Z2022 Oct2025-04-18T21:22:31.065Z2025-04-07T09:17:32.018ZS-EPMC95964903628409110.1038/s41467-022-33203-1