biostudies-literatureZhu ZNational Natural Science Foundation of ChinaNatural Science Foundation of Jiangsu Province750-758https://www.ebi.ac.uk/biostudies/studies/S-EPMC9597435biostudies-literatureUnknown46(6)<h4>Background</h4>Mild cognitive impairment (MCI) is a transitional condition between normality and dementia. Ginseng is known to have effects on attenuating cognitive deficits in neurogenerative diseases. Ginsenosides are the main bioactive component of ginseng, and their protein targets have not been fully understood. Furthermore, no thorough analysis is reported in ginsenoside-related protein targets in MCI.<h4>Methods</h4>The candidate protein targets of ginsenosides in brain tissues were identified by drug affinity responsive target stability (DARTS) coupled with label-free liquid chromatography-mass spectrometry (LC-MS) analysis. Network pharmacology approach was used to collect the therapeutic targets for MCI. Based on the above-mentioned overlapping targets, we built up a protein-Journal of ginseng researchTarget engagement of ginsenosides in mild cognitive impairment using mass spectrometry-based drug affinity responsive target stability.PMC959743582001418588200397081873025BK20181423Zhu ZZhang HChen LChen FLi RQin WChen CCheng YZhao YfalseTarget engagement of ginsenosides in mild cognitive impairment using mass spectrometry-based drug affinity responsive target stability.<h4>Background</h4>Mild cognitive impairment (MCI) is a transitional condition between normality and dementia. Ginseng is known to have effects on attenuating cognitive deficits in neurogenerative diseases. Ginsenosides are the main bioactive component of ginseng, and their protein targets have not been fully understood. Furthermore, no thorough analysis is reported in ginsenoside-related protein targets in MCI.<h4>Methods</h4>The candidate protein targets of ginsenosides in brain tissues were identified by drug affinity responsive target stability (DARTS) coupled with label-free liquid chromatography-mass spectrometry (LC-MS) analysis. Network pharmacology approach was used to collect the therapeutic targets for MCI. Based on the above-mentioned overlapping targets, we built up a protein-2022-01-01T00:00:00Z2022 Nov2026-06-01T02:25:57.107Z2025-04-07T09:18:09.609ZS-EPMC95974353631273410.1016/j.jgr.2021.12.003