biostudies-literatureUnknown13Kasarla SSAyurvedic medicines <i>Withania somnifera</i> Dunal (<i>ashwagandha</i>) and AYUSH-64 have been used for the prevention and management of COVID-19 in India. The present study explores the effect of <i>Ashwagandha</i> and AYUSH-64 on important human CYP enzymes (CYP3A4, CYP2C8, and CYP2D6) to assess their interaction with remdesivir, a drug used for COVID-19 management during the second wave. The study also implies possible herb-drug interactions as <i>ashwagandha</i> and AYUSH-64 are being used for managing various pathological conditions. Aqueous extracts of <i>ashwagandha</i> and AYUSH-64 were characterized using LC-MS/MS. A total of 11 and 24 phytoconstituents were identified putatively from <i>ashwagandha</i> and AYUSH-64 extracts, respectively. In addition, <i>in silico</i> studies reFrontiers in pharmacology973768https://www.ebi.ac.uk/biostudies/studies/S-EPMC9597875biostudies-literature<i>In vitro</i> effect of <i>Withania somnifera</i>, AYUSH-64, and remdesivir on the activity of CYP-450 enzymes: Implications for possible herb-drug interactions in the management of COVID-19.PMC9597875Kasarla SSSharma NKumar YDikshit MBorse SPfalse<i>In vitro</i> effect of <i>Withania somnifera</i>, AYUSH-64, and remdesivir on the activity of CYP-450 enzymes: Implications for possible herb-drug interactions in the management of COVID-19.Ayurvedic medicines <i>Withania somnifera</i> Dunal (<i>ashwagandha</i>) and AYUSH-64 have been used for the prevention and management of COVID-19 in India. The present study explores the effect of <i>Ashwagandha</i> and AYUSH-64 on important human CYP enzymes (CYP3A4, CYP2C8, and CYP2D6) to assess their interaction with remdesivir, a drug used for COVID-19 management during the second wave. The study also implies possible herb-drug interactions as <i>ashwagandha</i> and AYUSH-64 are being used for managing various pathological conditions. Aqueous extracts of <i>ashwagandha</i> and AYUSH-64 were characterized using LC-MS/MS. A total of 11 and 24 phytoconstituents were identified putatively from <i>ashwagandha</i> and AYUSH-64 extracts, respectively. In addition, <i>in silico</i> studies re2022-01-01T00:00:00Z20222026-04-08T11:38:07.675Z2024-11-09T20:37:10.027ZS-EPMC95978753631331310.3389/fphar.2022.973768