{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Luo M"],"funding":["CAMS Innovation Fund for Medical Sciences","National Natural Science Foundation of China","The National Natural Science Foundation of China"],"pagination":["1304"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9598291"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(10)"],"pubmed_abstract":["Genome mining has become an important tool for discovering new natural products and identifying the cryptic biosynthesis gene clusters. Here, we utilized the flavin-dependent halogenase GedL as the probe in combination with characteristic halogen isotope patterns to mine new halogenated secondary metabolites from our in-house fungal database. As a result, two pairs of atropisomers, pestalachlorides A1a (1a)/A1b (1b) and A2a (2a)/A2b (2b), along with known compounds pestalachloride A (3) and SB87-H (4), were identified from Pestalotiopsis rhododendri LF-19-12. A plausible biosynthetic assembly line for pestalachlorides involving a putative free-standing phenol flavin-dependent halogenase was proposed based on bioinformatics analysis. Pestalachlorides exhibited antibacterial activity against"],"journal":["Antibiotics (Basel, Switzerland)"],"pubmed_title":["Halogenase-Targeted Genome Mining Leads to the Discovery of (±) Pestalachlorides A1a, A2a, and Their Atropisomers."],"pmcid":["PMC9598291"],"funding_grant_id":["81973219 and 82104047","CIFMS, 2021-I2M-1-028","81973219","82104047"],"pubmed_authors":["Wang M","Shan G","Xie Y","Luo M","He N","Chang S"],"additional_accession":[]},"is_claimable":false,"name":"Halogenase-Targeted Genome Mining Leads to the Discovery of (±) Pestalachlorides A1a, A2a, and Their Atropisomers.","description":"Genome mining has become an important tool for discovering new natural products and identifying the cryptic biosynthesis gene clusters. Here, we utilized the flavin-dependent halogenase GedL as the probe in combination with characteristic halogen isotope patterns to mine new halogenated secondary metabolites from our in-house fungal database. As a result, two pairs of atropisomers, pestalachlorides A1a (1a)/A1b (1b) and A2a (2a)/A2b (2b), along with known compounds pestalachloride A (3) and SB87-H (4), were identified from Pestalotiopsis rhododendri LF-19-12. A plausible biosynthetic assembly line for pestalachlorides involving a putative free-standing phenol flavin-dependent halogenase was proposed based on bioinformatics analysis. Pestalachlorides exhibited antibacterial activity against","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2026-06-20T03:25:23.105Z","creation":"2025-04-07T09:15:19.476Z"},"accession":"S-EPMC9598291","cross_references":{"pubmed":["36289962"],"doi":["10.3390/antibiotics11101304"]}}