{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(10)"],"submitter":["Gonzalo X"],"pubmed_abstract":["We evaluated a novel physiological 3-D bioelectrospray model of the tuberculosis (TB) granuloma to test the activity of a known anti-TB drug, clofazimine; three carbapenems with potential activity, including one currently used in therapy; and nitazoxanide, an anti-parasitic compound with possible TB activity (all chosen as conventional drug susceptibility was problematical). PBMCs collected from healthy donors were isolated and infected with <i>M. tuberculosis</i> H37Rv lux (i.e., luciferase). Microspheres were generated with the infected cells; the anti-microbial compounds were added and bacterial luminescence was monitored for at least 21 days. Clavulanate was added to each carbapenem to inhibit beta-lactamases. <i>M. tuberculosis</i> (MTB) killing efficacy was dose dependent. Clofazimin"],"journal":["Antibiotics (Basel, Switzerland)"],"pagination":["1274"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9598577"],"repository":["biostudies-literature"],"pubmed_title":["Anti-Tuberculosis Activity of Three Carbapenems, Clofazimine and Nitazoxanide Using a Novel Ex Vivo Phenotypic Drug Susceptibility Model of Human Tuberculosis."],"pmcid":["PMC9598577"],"pubmed_authors":["Bielecka MK","Elkington P","Drobniewski F","Gonzalo X","Tezera L"],"additional_accession":[]},"is_claimable":false,"name":"Anti-Tuberculosis Activity of Three Carbapenems, Clofazimine and Nitazoxanide Using a Novel Ex Vivo Phenotypic Drug Susceptibility Model of Human Tuberculosis.","description":"We evaluated a novel physiological 3-D bioelectrospray model of the tuberculosis (TB) granuloma to test the activity of a known anti-TB drug, clofazimine; three carbapenems with potential activity, including one currently used in therapy; and nitazoxanide, an anti-parasitic compound with possible TB activity (all chosen as conventional drug susceptibility was problematical). PBMCs collected from healthy donors were isolated and infected with <i>M. tuberculosis</i> H37Rv lux (i.e., luciferase). Microspheres were generated with the infected cells; the anti-microbial compounds were added and bacterial luminescence was monitored for at least 21 days. Clavulanate was added to each carbapenem to inhibit beta-lactamases. <i>M. tuberculosis</i> (MTB) killing efficacy was dose dependent. Clofazimin","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2025-04-04T11:29:26.49Z","creation":"2025-04-04T11:29:26.49Z"},"accession":"S-EPMC9598577","cross_references":{"pubmed":["36289932"],"doi":["10.3390/antibiotics11101274"]}}