biostudies-literatureUnknown11(10)Gonzalo XWe evaluated a novel physiological 3-D bioelectrospray model of the tuberculosis (TB) granuloma to test the activity of a known anti-TB drug, clofazimine; three carbapenems with potential activity, including one currently used in therapy; and nitazoxanide, an anti-parasitic compound with possible TB activity (all chosen as conventional drug susceptibility was problematical). PBMCs collected from healthy donors were isolated and infected with <i>M. tuberculosis</i> H37Rv lux (i.e., luciferase). Microspheres were generated with the infected cells; the anti-microbial compounds were added and bacterial luminescence was monitored for at least 21 days. Clavulanate was added to each carbapenem to inhibit beta-lactamases. <i>M. tuberculosis</i> (MTB) killing efficacy was dose dependent. ClofaziminAntibiotics (Basel, Switzerland)1274https://www.ebi.ac.uk/biostudies/studies/S-EPMC9598577biostudies-literatureAnti-Tuberculosis Activity of Three Carbapenems, Clofazimine and Nitazoxanide Using a Novel Ex Vivo Phenotypic Drug Susceptibility Model of Human Tuberculosis.PMC9598577Bielecka MKElkington PDrobniewski FGonzalo XTezera LfalseAnti-Tuberculosis Activity of Three Carbapenems, Clofazimine and Nitazoxanide Using a Novel Ex Vivo Phenotypic Drug Susceptibility Model of Human Tuberculosis.We evaluated a novel physiological 3-D bioelectrospray model of the tuberculosis (TB) granuloma to test the activity of a known anti-TB drug, clofazimine; three carbapenems with potential activity, including one currently used in therapy; and nitazoxanide, an anti-parasitic compound with possible TB activity (all chosen as conventional drug susceptibility was problematical). PBMCs collected from healthy donors were isolated and infected with <i>M. tuberculosis</i> H37Rv lux (i.e., luciferase). Microspheres were generated with the infected cells; the anti-microbial compounds were added and bacterial luminescence was monitored for at least 21 days. Clavulanate was added to each carbapenem to inhibit beta-lactamases. <i>M. tuberculosis</i> (MTB) killing efficacy was dose dependent. Clofazimin2022-01-01T00:00:00Z2022 Sep2025-04-04T11:29:26.49Z2025-04-04T11:29:26.49ZS-EPMC95985773628993210.3390/antibiotics11101274