biostudies-literatureLejars MMEXT | Japan Society for the Promotion of ScienceAgence Nationale de la RechercheCentre National de la Recherche Scientifiquee0098122https://www.ebi.ac.uk/biostudies/studies/S-EPMC9600491biostudies-literatureUnknown13(5)In order to respond to ever-changing environmental cues, bacteria display resilient regulatory mechanisms controlling gene expression. At the post-transcriptional level, this is achieved by a combination of RNA-binding proteins, such as ribonucleases (RNases), and regulatory RNAs, including antisense RNAs (asRNAs). Bound to their complementary mRNA, asRNAs are primary targets for the double-strand-specific endoribonuclease, RNase III. Taking advantage of our own and previously published transcriptomic data sets obtained in strains inactivated for RNase III, we selected several candidate asRNAs and confirmed the existence of RNase III-sensitive asRNAs for <i>crp</i>, <i>ompR</i>, <i>phoP</i>, and <i>flhD</i> genes, all encoding global regulators of gene expression in Escherichia coli. UsingmBioRegulatory Interplay between RNase III and Antisense RNAs in E. coli: the Case of AsflhD and FlhD, Component of the Master Regulator of Motility.PMC9600491&quot;DYNAMO &quot; ANR-11-LABX-0011P22709asSUPYCO ANR-12-BSV6-0007-03RIBECO ANR-18-CE43-0010Lejars MCaillet JSolchaga-Flores EHajnsdorf EGuillier MPlumbridge JfalseRegulatory Interplay between RNase III and Antisense RNAs in E. coli: the Case of AsflhD and FlhD, Component of the Master Regulator of Motility.In order to respond to ever-changing environmental cues, bacteria display resilient regulatory mechanisms controlling gene expression. At the post-transcriptional level, this is achieved by a combination of RNA-binding proteins, such as ribonucleases (RNases), and regulatory RNAs, including antisense RNAs (asRNAs). Bound to their complementary mRNA, asRNAs are primary targets for the double-strand-specific endoribonuclease, RNase III. Taking advantage of our own and previously published transcriptomic data sets obtained in strains inactivated for RNase III, we selected several candidate asRNAs and confirmed the existence of RNase III-sensitive asRNAs for <i>crp</i>, <i>ompR</i>, <i>phoP</i>, and <i>flhD</i> genes, all encoding global regulators of gene expression in Escherichia coli. Using2022-01-01T00:00:00Z2022 Oct2026-07-14T15:22:53.459Z2024-11-12T17:34:35.343ZS-EPMC96004913600073310.1128/mbio.00981-22