{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["44(10)"],"submitter":["Orlandi E"],"pubmed_abstract":["Polymorphisms in the ribonuclease L (RNASEL) coding gene and hsa-miR-146a-5p (miR-146a) have been associated with melanoma in a sex-specific manner. We hypothesized that RNASEL and miR-146a expression could be influenced by sex hormones playing a role in the female advantages observed in melanoma incidence and survival. Thus, we explored the effects of testosterone and 17β-estradiol on RNASEL and miR-146a expression in LM-20 and A375 melanoma cell lines. Direct targeting of miR-146a to the 3' untranslated region (3'UTR) of RNASEL was examined using a luciferase reporter system. Our results indicate that RNASEL is a direct target of miR-146a in both melanoma cell lines. Trough qPCR and western blot analyses, we explored the effect of miR-146a mimic transfection in the presence of each hormo"],"journal":["Current issues in molecular biology"],"pagination":["4790-4802"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9601115"],"repository":["biostudies-literature"],"pubmed_title":["Human Melanoma Cells Differentially Express RNASEL/RNase-L and miR-146a-5p under Sex Hormonal Stimulation."],"pmcid":["PMC9601115"],"pubmed_authors":["Belpinati F","Campagnari R","Rodolfo M","Gomez-Lira M","Menegazzi M","Vergani E","Orlandi E","De Tomi E","Malerba G","Romanelli MG"],"additional_accession":[]},"is_claimable":false,"name":"Human Melanoma Cells Differentially Express RNASEL/RNase-L and miR-146a-5p under Sex Hormonal Stimulation.","description":"Polymorphisms in the ribonuclease L (RNASEL) coding gene and hsa-miR-146a-5p (miR-146a) have been associated with melanoma in a sex-specific manner. We hypothesized that RNASEL and miR-146a expression could be influenced by sex hormones playing a role in the female advantages observed in melanoma incidence and survival. Thus, we explored the effects of testosterone and 17β-estradiol on RNASEL and miR-146a expression in LM-20 and A375 melanoma cell lines. Direct targeting of miR-146a to the 3' untranslated region (3'UTR) of RNASEL was examined using a luciferase reporter system. Our results indicate that RNASEL is a direct target of miR-146a in both melanoma cell lines. Trough qPCR and western blot analyses, we explored the effect of miR-146a mimic transfection in the presence of each hormo","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-05-31T23:54:54.585Z","creation":"2024-11-12T17:34:30.469Z"},"accession":"S-EPMC9601115","cross_references":{"pubmed":["36286041"],"doi":["10.3390/cimb44100326"]}}