biostudies-literatureUnknown23(20)Rahman MAThe Gram-positive bacterium <i>Staphylococcus aureus</i> is responsible for serious acute and chronic infections worldwide and is well-known for its biofilm formation ability. Recent findings of biofilms on dry hospital surfaces emphasise the failures in current cleaning practices and disinfection and the difficulty in removing these dry surface biofilms (DSBs). Many aspects of the formation of complex DSB biology on environmental surfaces in healthcare settings remains limited. In the present study, we aimed to determine how the protein component varied between DSBs and traditional hydrated biofilm. To do this, biofilms were grown in tryptic soy broth (TSB) on removable polycarbonate coupons in the CDC biofilm reactor over 12 days. Hydrated biofilm (50% TSB for 48 h, the media was then chInternational journal of molecular sciences12238https://www.ebi.ac.uk/biostudies/studies/S-EPMC9602640biostudies-literatureOne Step Forward with Dry Surface Biofilm (DSB) of <i>Staphylococcus aureus</i>: TMT-Based Quantitative Proteomic Analysis Reveals Proteomic Shifts between DSB and Hydrated Biofilm.PMC9602640Amirkhani AMolloy MPVickery KDeva AKParvin FHu HChowdhury DRahman MAfalseOne Step Forward with Dry Surface Biofilm (DSB) of <i>Staphylococcus aureus</i>: TMT-Based Quantitative Proteomic Analysis Reveals Proteomic Shifts between DSB and Hydrated Biofilm.The Gram-positive bacterium <i>Staphylococcus aureus</i> is responsible for serious acute and chronic infections worldwide and is well-known for its biofilm formation ability. Recent findings of biofilms on dry hospital surfaces emphasise the failures in current cleaning practices and disinfection and the difficulty in removing these dry surface biofilms (DSBs). Many aspects of the formation of complex DSB biology on environmental surfaces in healthcare settings remains limited. In the present study, we aimed to determine how the protein component varied between DSBs and traditional hydrated biofilm. To do this, biofilms were grown in tryptic soy broth (TSB) on removable polycarbonate coupons in the CDC biofilm reactor over 12 days. Hydrated biofilm (50% TSB for 48 h, the media was then ch2022-01-01T00:00:00Z2022 Oct2026-06-20T03:20:24.807Z2024-11-13T23:30:54.629ZS-EPMC96026403629309210.3390/ijms232012238