{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["23(20)"],"submitter":["Ke J"],"pubmed_abstract":["In recent years, cyclic peptides have attracted much attention due to their chemical and enzymatic stability, low toxicity, and easy modification. In general, the self-assembled nanostructures of cyclic peptides tend to form nanotubes in a cyclic stacking manner through hydrogen bonding. However, studies exploring other assembly strategies are scarce. In this context, we proposed a new assembly strategy based on cyclic peptides with covalent self-assembly. Here, cyclic peptide-(DPDPDP) was rationally designed and used as a building block to construct new assemblies. With cyclo-(DP)<sub>3</sub> as the structural unit and 2,2'-diamino-N-methyldiethylamine as the linker, positively charged nanospheres ((CP)<sub>6</sub>NS) based on cyclo-(DP)<sub>3</sub> were successfully constructed by covalent self-assembly. We assessed their size and morphology by scanning electron microscopy (SEM), TEM, and DLS. (CP)<sub>6</sub>NS were found to have a strong positive charge, so they could bind to siRNA through electrostatic interactions. Confocal microscopy analysis and cell viability assays showed that (CP)<sub>6</sub>NS had high cellular internalization efficiency and low cytotoxicity. More importantly, real-time polymerase chain reaction (PCR) and flow cytometry analyses indicated that (CP)<sub>6</sub>NS-siRNA complexes potently inhibited gene expression and promoted tumor cell apoptosis. These results suggest that (CP)<sub>6</sub>NS may be a potential siRNA carrier for gene therapy."],"journal":["International journal of molecular sciences"],"pagination":["12071"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9602810"],"repository":["biostudies-literature"],"pubmed_title":["Design of Cyclic Peptide-Based Nanospheres and the Delivery of siRNA."],"pmcid":["PMC9602810"],"pubmed_authors":["Liu J","Li J","Zhang J","Ke J","Guan S"],"additional_accession":[]},"is_claimable":false,"name":"Design of Cyclic Peptide-Based Nanospheres and the Delivery of siRNA.","description":"In recent years, cyclic peptides have attracted much attention due to their chemical and enzymatic stability, low toxicity, and easy modification. In general, the self-assembled nanostructures of cyclic peptides tend to form nanotubes in a cyclic stacking manner through hydrogen bonding. However, studies exploring other assembly strategies are scarce. In this context, we proposed a new assembly strategy based on cyclic peptides with covalent self-assembly. Here, cyclic peptide-(DPDPDP) was rationally designed and used as a building block to construct new assemblies. With cyclo-(DP)<sub>3</sub> as the structural unit and 2,2'-diamino-N-methyldiethylamine as the linker, positively charged nanospheres ((CP)<sub>6</sub>NS) based on cyclo-(DP)<sub>3</sub> were successfully constructed by covalent self-assembly. We assessed their size and morphology by scanning electron microscopy (SEM), TEM, and DLS. (CP)<sub>6</sub>NS were found to have a strong positive charge, so they could bind to siRNA through electrostatic interactions. Confocal microscopy analysis and cell viability assays showed that (CP)<sub>6</sub>NS had high cellular internalization efficiency and low cytotoxicity. More importantly, real-time polymerase chain reaction (PCR) and flow cytometry analyses indicated that (CP)<sub>6</sub>NS-siRNA complexes potently inhibited gene expression and promoted tumor cell apoptosis. These results suggest that (CP)<sub>6</sub>NS may be a potential siRNA carrier for gene therapy.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-03T21:29:44.878Z","creation":"2025-04-03T21:29:44.878Z"},"accession":"S-EPMC9602810","cross_references":{"pubmed":["36292932"],"doi":["10.3390/ijms232012071"]}}