{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(5)"],"submitter":["Hazu M"],"pubmed_abstract":["The isolation of pure, single colonies lies at the heart of experimental microbiology. However, a microbial colony typically contains around 1 million cells at all stages of the life cycle. Here, we describe a novel cell chromatography method that facilitates the capture, purification, and interrogation of microbial cell populations from both single and mixed cultures. The method described relies on, but is not limited to, differences in surface charge to separate bacterial strains. The method is fully biocompatible, leading to no significant loss of cell viability. The chromatographic capture of cells, combined with selective elution methods, facilitates a greater level of experimental control over the sample inputs required for downstream high-throughput and high-sensitivity analytical m"],"journal":["Microbiology spectrum"],"pagination":["e0245022"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9603491"],"repository":["biostudies-literature"],"pubmed_title":["Cell Chromatography: Biocompatible Chromatographic Separation and Interrogation of Microbial Cells."],"pmcid":["PMC9603491"],"pubmed_authors":["Curry E","Ahmed A","Hornby DP","Gjerde DT","Hazu M"],"additional_accession":[]},"is_claimable":false,"name":"Cell Chromatography: Biocompatible Chromatographic Separation and Interrogation of Microbial Cells.","description":"The isolation of pure, single colonies lies at the heart of experimental microbiology. However, a microbial colony typically contains around 1 million cells at all stages of the life cycle. Here, we describe a novel cell chromatography method that facilitates the capture, purification, and interrogation of microbial cell populations from both single and mixed cultures. The method described relies on, but is not limited to, differences in surface charge to separate bacterial strains. The method is fully biocompatible, leading to no significant loss of cell viability. The chromatographic capture of cells, combined with selective elution methods, facilitates a greater level of experimental control over the sample inputs required for downstream high-throughput and high-sensitivity analytical m","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-03T21:29:13.851Z","creation":"2025-04-03T21:29:13.851Z"},"accession":"S-EPMC9603491","cross_references":{"pubmed":["36129286"],"doi":["10.1128/spectrum.02450-22"]}}