biostudies-literatureChudakov DBRussian Foundation for Basic Research13063https://www.ebi.ac.uk/biostudies/studies/S-EPMC9603618biostudies-literatureUnknown19(20)<h4>Background</h4>Diesel exhaust particles (DEPs) are leading to a general increase in atopic diseases worldwide. However, it is still unknown whether DEPs induce systemic B-cell IgE class switching in secondary lymphoid organs or locally in the lungs in inducible bronchus-associated lymphoid tissue (iBALT). The aim of this work was to identify the exact site of DEP-mediated B-cell IgE class switching and pro-allergic antibody production.<h4>Methods</h4>We immunized BALB/c mice with different OVA doses (0.3 and 30 µg) intranasally in the presence and absence of two types of DEPs, SRM1650B and SRM2786. We used low (30 µg) and high (150 µg) DEP doses.<h4>Results</h4>Only a high DEP dose induced IgE production, regardless of the particle type. Local IgE class switching was stimulated upon trInternational journal of environmental research and public healthDEPs Induce Local Ige Class Switching Independent of Their Ability to Stimulate iBALT de Novo Formation.PMC960361819-05-5006420-015-00360Shevchenko MAKonovalova MVTsaregorodtseva DSChudakov DBKashirina EIFattakhova GVKotsareva ODfalseDEPs Induce Local Ige Class Switching Independent of Their Ability to Stimulate iBALT de Novo Formation.<h4>Background</h4>Diesel exhaust particles (DEPs) are leading to a general increase in atopic diseases worldwide. However, it is still unknown whether DEPs induce systemic B-cell IgE class switching in secondary lymphoid organs or locally in the lungs in inducible bronchus-associated lymphoid tissue (iBALT). The aim of this work was to identify the exact site of DEP-mediated B-cell IgE class switching and pro-allergic antibody production.<h4>Methods</h4>We immunized BALB/c mice with different OVA doses (0.3 and 30 µg) intranasally in the presence and absence of two types of DEPs, SRM1650B and SRM2786. We used low (30 µg) and high (150 µg) DEP doses.<h4>Results</h4>Only a high DEP dose induced IgE production, regardless of the particle type. Local IgE class switching was stimulated upon tr2022-01-01T00:00:00Z2022 Oct2025-04-03T21:30:28.437Z2025-04-03T21:30:28.437ZS-EPMC96036183629364210.3390/ijerph192013063