biostudies-literatureUnknownChen NFGNCATS NIH HHSThe 2022 multi-country monkeypox (mpox) outbreak concurrent with the ongoing COVID-19 pandemic has further highlighted the need for genomic surveillance and rapid pathogen whole genome sequencing. While metagenomic sequencing approaches have been used to sequence many of the early mpox infections, these methods are resource intensive and require samples with high viral DNA concentrations. Given the atypical clinical presentation of cases associated with the outbreak and uncertainty regarding viral load across both the course of infection and anatomical body sites, there was an urgent need for a more sensitive and broadly applicable sequencing approach. Highly multiplexed amplicon-based sequencing (PrimalSeq) was initially developed for sequencing of Zika virus, and later adapted as the maimedRxiv : the preprint server for health sciences2022.10.14.22280783https://www.ebi.ac.uk/biostudies/studies/S-EPMC9603838biostudies-literatureDevelopment of an amplicon-based sequencing approach in response to the global emergence of human monkeypox virus.PMC9603838UL1 TR001863Fall AGallagher GRBorges VHuard RCPlumb MRBarcellos RBSevinsky JBreban MIGreen NMBajwa MDuarte SGagnon EGrubaugh NDVogels CBFCotton SNaumann AKapsak CJWang XChurchwell GMathers KHemarajata PDewar RHall JHayward NMostafa HHReever EPark DJCofsky SParker AChen NFGSantos DGomes JPFelton KSierra-Patev SVieira LTempleton KEAsh SMcGruder BBuzby EMiller HKenicer JRazeq JSalvato RSLee PAClancy SCeniseros AHarrington RFerreira RMcHugh MPDoucette MSmole SMuyombwe ASobral DLibuit KPedrosa MCarpenter-Azevedo KAquino CSchmedes SHuang JPPearson CNishimura CSavino IMAllman RMaloney DMGagne LIncekara KGreer NChaguza CGodinho FMSGarrigues JMWild JfalseDevelopment of an amplicon-based sequencing approach in response to the global emergence of human monkeypox virus.The 2022 multi-country monkeypox (mpox) outbreak concurrent with the ongoing COVID-19 pandemic has further highlighted the need for genomic surveillance and rapid pathogen whole genome sequencing. While metagenomic sequencing approaches have been used to sequence many of the early mpox infections, these methods are resource intensive and require samples with high viral DNA concentrations. Given the atypical clinical presentation of cases associated with the outbreak and uncertainty regarding viral load across both the course of infection and anatomical body sites, there was an urgent need for a more sensitive and broadly applicable sequencing approach. Highly multiplexed amplicon-based sequencing (PrimalSeq) was initially developed for sequencing of Zika virus, and later adapted as the mai2023-01-01T00:00:00Z2023 Jan2026-04-08T11:44:15.035Z2025-04-06T12:12:01.668ZS-EPMC96038383629942010.1101/2022.10.14.22280783