biostudies-literatureUnknown28Zehra Z<h4>Purpose</h4>Strabismus (STBMS) is a multifactorial ocular disorder in children that leads to misalignment of the eyes. Insulin-like growth factor 1 (<i>IGF1</i>) has been shown to be involved in the development of extraocular muscles and myopia; however, data are limited on the genetic associations of <i>IGF1</i> with STBMS in Pakistan.<h4>Methods</h4>Two hundred seventy-four STBMS cases and 272 unaffected controls were recruited, and their DNA was extracted. Two <i>IGF1</i> single nucleotide polymorphisms, rs6214 and rs5742632, were genotyped using PCR-restriction fragment length polymorphism. Univariate logistic regression analysis was performed to determine the association of these single nucleotide polymorphisms with STBMS, and the results were adjusted for age and sex. In additionMolecular vision369-377https://www.ebi.ac.uk/biostudies/studies/S-EPMC9603902biostudies-literatureAssociation of <i>IGF1</i> polymorphisms with exotropia in a Pakistani cohort.PMC9603902Siddiqui SNNadeem Mvon Bartheld CSKhan NQamar RAzam MZehra ZfalseAssociation of <i>IGF1</i> polymorphisms with exotropia in a Pakistani cohort.<h4>Purpose</h4>Strabismus (STBMS) is a multifactorial ocular disorder in children that leads to misalignment of the eyes. Insulin-like growth factor 1 (<i>IGF1</i>) has been shown to be involved in the development of extraocular muscles and myopia; however, data are limited on the genetic associations of <i>IGF1</i> with STBMS in Pakistan.<h4>Methods</h4>Two hundred seventy-four STBMS cases and 272 unaffected controls were recruited, and their DNA was extracted. Two <i>IGF1</i> single nucleotide polymorphisms, rs6214 and rs5742632, were genotyped using PCR-restriction fragment length polymorphism. Univariate logistic regression analysis was performed to determine the association of these single nucleotide polymorphisms with STBMS, and the results were adjusted for age and sex. In addition2022-01-01T00:00:00Z20222025-06-01T03:59:33.12Z2024-11-19T17:04:55.069ZS-EPMC960390236338665