{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yeo NJY"],"funding":["Ministry of Education","Nanyang Technological University","Agency for Science, Technology and Research"],"pagination":["12549"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9604336"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(20)"],"pubmed_abstract":["Age-related macular degeneration (AMD) is a global leading cause of visual impairment in older populations. 'Wet' AMD, the most common subtype of this disease, occurs when pathological angiogenesis infiltrates the subretinal space (choroidal neovascularization), causing hemorrhage and retinal damage. Gold standard anti-vascular endothelial growth factor (VEGF) treatment is an effective therapy, but the long-term prevention of visual decline has not been as successful. This warrants the need to elucidate potential VEGF-independent pathways. We generated blood out-growth endothelial cells (BOECs) from wet AMD and normal control subjects, then induced angiogenic sprouting of BOECs using a fibrin gel bead assay. To deconvolute endothelial heterogeneity, we performed single-cell transcriptomic "],"journal":["International journal of molecular sciences"],"pubmed_title":["Single-Cell Transcriptome of Wet AMD Patient-Derived Endothelial Cells in Angiogenic Sprouting."],"pmcid":["PMC9604336"],"funding_grant_id":["MOE2018-T2-1-042","SPF2014/002","Nanyang Assistant Professorship"],"pubmed_authors":["Wu KX","Cheung C","Nguyen NLU","Ng CY","Fan Q","Wazny V","Cheung CMG","Yeo NJY"],"additional_accession":[]},"is_claimable":false,"name":"Single-Cell Transcriptome of Wet AMD Patient-Derived Endothelial Cells in Angiogenic Sprouting.","description":"Age-related macular degeneration (AMD) is a global leading cause of visual impairment in older populations. 'Wet' AMD, the most common subtype of this disease, occurs when pathological angiogenesis infiltrates the subretinal space (choroidal neovascularization), causing hemorrhage and retinal damage. Gold standard anti-vascular endothelial growth factor (VEGF) treatment is an effective therapy, but the long-term prevention of visual decline has not been as successful. This warrants the need to elucidate potential VEGF-independent pathways. We generated blood out-growth endothelial cells (BOECs) from wet AMD and normal control subjects, then induced angiogenic sprouting of BOECs using a fibrin gel bead assay. To deconvolute endothelial heterogeneity, we performed single-cell transcriptomic ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-19T12:05:11.195Z","creation":"2025-04-19T12:05:11.195Z"},"accession":"S-EPMC9604336","cross_references":{"pubmed":["36293401"],"doi":["10.3390/ijms232012549"]}}