{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li Y"],"funding":["Key Research Program of Frontier Sciences, Chinese Academy of Sciences","Biological Resources Program, Chinese Academy of Sciences","National Natural Science Foundation of China","China Postdoctoral Science Foundation","National Key Research and Development Program of China"],"pagination":["1001"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9605436"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(10)"],"pubmed_abstract":["Nonribosomal peptide synthetase (NRPS)-like enzymes containing A-T-R domain architecture are also known as carboxylate reductases (CARs) for aldehyde generation. To identify new members of CARs, we established a virtual library containing 84 fungal CARs distributed in seven distinct clades by genome mining and phylogenetic analysis. Nine CARs, including PnlA from <i>Pestalotiopsis fici</i> and eight known CARs, were clustered in clade VI and proposed to catalyze the reduction of nonreducing polyketide synthase (NR-PKS)-derived aryl carboxylic acids. The recombinant protein PnlA was overproduced and purified to apparent homogeneity from <i>Saccharomyces cerevisiae</i>. In vitro enzyme assays of PnlA with 28 different benzoic acid derivatives (<b>1</b>-<b>28</b>) revealed the corresponding a"],"journal":["Journal of fungi (Basel, Switzerland)"],"pubmed_title":["Characterization of a NRPS-like Protein from <i>Pestalotiopsis fici</i> for Aldehyde Generation."],"pmcid":["PMC9605436"],"funding_grant_id":["KFJ-BRP-009-005","31861133004","2022T150689","YJ20200201","2021M693362","YJ20200309","ZDBS-LY-SM016","2020YFA0907800","2020M680720"],"pubmed_authors":["Li Y","Wei PL","Yin WB","Fan J","Ran H"],"additional_accession":[]},"is_claimable":false,"name":"Characterization of a NRPS-like Protein from <i>Pestalotiopsis fici</i> for Aldehyde Generation.","description":"Nonribosomal peptide synthetase (NRPS)-like enzymes containing A-T-R domain architecture are also known as carboxylate reductases (CARs) for aldehyde generation. To identify new members of CARs, we established a virtual library containing 84 fungal CARs distributed in seven distinct clades by genome mining and phylogenetic analysis. Nine CARs, including PnlA from <i>Pestalotiopsis fici</i> and eight known CARs, were clustered in clade VI and proposed to catalyze the reduction of nonreducing polyketide synthase (NR-PKS)-derived aryl carboxylic acids. The recombinant protein PnlA was overproduced and purified to apparent homogeneity from <i>Saccharomyces cerevisiae</i>. In vitro enzyme assays of PnlA with 28 different benzoic acid derivatives (<b>1</b>-<b>28</b>) revealed the corresponding a","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2025-04-19T12:07:57.636Z","creation":"2025-04-19T12:07:57.636Z"},"accession":"S-EPMC9605436","cross_references":{"pubmed":["36294566"],"doi":["10.3390/jof8101001"]}}