biostudies-literatureLi YKey Research Program of Frontier Sciences, Chinese Academy of SciencesBiological Resources Program, Chinese Academy of SciencesNational Natural Science Foundation of ChinaChina Postdoctoral Science FoundationNational Key Research and Development Program of China1001https://www.ebi.ac.uk/biostudies/studies/S-EPMC9605436biostudies-literatureUnknown8(10)Nonribosomal peptide synthetase (NRPS)-like enzymes containing A-T-R domain architecture are also known as carboxylate reductases (CARs) for aldehyde generation. To identify new members of CARs, we established a virtual library containing 84 fungal CARs distributed in seven distinct clades by genome mining and phylogenetic analysis. Nine CARs, including PnlA from <i>Pestalotiopsis fici</i> and eight known CARs, were clustered in clade VI and proposed to catalyze the reduction of nonreducing polyketide synthase (NR-PKS)-derived aryl carboxylic acids. The recombinant protein PnlA was overproduced and purified to apparent homogeneity from <i>Saccharomyces cerevisiae</i>. In vitro enzyme assays of PnlA with 28 different benzoic acid derivatives (<b>1</b>-<b>28</b>) revealed the corresponding aJournal of fungi (Basel, Switzerland)Characterization of a NRPS-like Protein from <i>Pestalotiopsis fici</i> for Aldehyde Generation.PMC9605436KFJ-BRP-009-005318611330042022T150689YJ202002012021M693362YJ20200309ZDBS-LY-SM0162020YFA09078002020M680720Li YWei PLYin WBFan JRan HfalseCharacterization of a NRPS-like Protein from <i>Pestalotiopsis fici</i> for Aldehyde Generation.Nonribosomal peptide synthetase (NRPS)-like enzymes containing A-T-R domain architecture are also known as carboxylate reductases (CARs) for aldehyde generation. To identify new members of CARs, we established a virtual library containing 84 fungal CARs distributed in seven distinct clades by genome mining and phylogenetic analysis. Nine CARs, including PnlA from <i>Pestalotiopsis fici</i> and eight known CARs, were clustered in clade VI and proposed to catalyze the reduction of nonreducing polyketide synthase (NR-PKS)-derived aryl carboxylic acids. The recombinant protein PnlA was overproduced and purified to apparent homogeneity from <i>Saccharomyces cerevisiae</i>. In vitro enzyme assays of PnlA with 28 different benzoic acid derivatives (<b>1</b>-<b>28</b>) revealed the corresponding a2022-01-01T00:00:00Z2022 Sep2025-04-19T12:07:57.636Z2025-04-19T12:07:57.636ZS-EPMC96054363629456610.3390/jof8101001