{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jaafar A"],"funding":["VEKOP","Ministry of Science and Higher Education of the Russian Federation","VISGEN","NEURAM"],"pagination":["1534"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9605590"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(10)"],"pubmed_abstract":["<i>Dura mater</i> (DM) is a connective tissue with dense collagen, which is a protective membrane surrounding the human brain. The optical clearing (OC) method was used to make DM more transparent, thereby allowing to increase in-depth investigation by confocal Raman micro-spectroscopy and estimate the diffusivity of 50% glycerol and water migration. Glycerol concentration was obtained, and the diffusion coefficient was calculated, which ranged from 9.6 × 10<sup>-6</sup> to 3.0 × 10<sup>-5</sup> cm<sup>2</sup>/s. Collagen-related Raman band intensities were significantly increased for all depths from 50 to 200 µm after treatment. In addition, the changes in water content during OC showed that 50% glycerol induces tissue dehydration. Weakly and strongly bound water types were found to be most concentrated, playing a major role in the glycerol-induced water flux and OC. Results show that OC is an efficient method for controlling the DM optical properties, thereby enhancing the in-depth probing for laser therapy and diagnostics of the brain. DM is a comparable to various collagen-containing tissues and organs, such as sclera of eyes and skin dermis."],"journal":["Life (Basel, Switzerland)"],"pubmed_title":["Confocal Raman Micro-Spectroscopy for Discrimination of Glycerol Diffusivity in Ex Vivo Porcine <i>Dura Mater</i>."],"pmcid":["PMC9605590"],"funding_grant_id":["2.3.2-16-2016-00011 and 2.3.2-16-2016-00002","075-15-2021-942","712821","VISGEN"],"pubmed_authors":["Darvin ME","Veres M","Jaafar A","Tuchin VV"],"additional_accession":[]},"is_claimable":false,"name":"Confocal Raman Micro-Spectroscopy for Discrimination of Glycerol Diffusivity in Ex Vivo Porcine <i>Dura Mater</i>.","description":"<i>Dura mater</i> (DM) is a connective tissue with dense collagen, which is a protective membrane surrounding the human brain. The optical clearing (OC) method was used to make DM more transparent, thereby allowing to increase in-depth investigation by confocal Raman micro-spectroscopy and estimate the diffusivity of 50% glycerol and water migration. Glycerol concentration was obtained, and the diffusion coefficient was calculated, which ranged from 9.6 × 10<sup>-6</sup> to 3.0 × 10<sup>-5</sup> cm<sup>2</sup>/s. Collagen-related Raman band intensities were significantly increased for all depths from 50 to 200 µm after treatment. In addition, the changes in water content during OC showed that 50% glycerol induces tissue dehydration. Weakly and strongly bound water types were found to be most concentrated, playing a major role in the glycerol-induced water flux and OC. Results show that OC is an efficient method for controlling the DM optical properties, thereby enhancing the in-depth probing for laser therapy and diagnostics of the brain. DM is a comparable to various collagen-containing tissues and organs, such as sclera of eyes and skin dermis.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-19T12:06:07.077Z","creation":"2025-04-19T12:06:07.077Z"},"accession":"S-EPMC9605590","cross_references":{"pubmed":["36294969"],"doi":["10.3390/life12101534"]}}