biostudies-literatureWu TKey-Area Research and Development Program of Guangdong Provincethe GDAS'Project of Science and Technology DevelopmentKey-Area Research and Development Program of Guangdong  Program of Guangdong Provincethe GDAS Project of Science and Technology Development1081https://www.ebi.ac.uk/biostudies/studies/S-EPMC9605614biostudies-literatureUnknown8(10)<i>Ganoderma lucidum</i> is an important medicinal mushroom widely cultured in Asian countries. Exopolysaccharides are bioactive compounds of <i>G. lucidum</i> with health benefits. Limited exopolysaccharide content hinders its extraction from <i>G. lucidum</i>. The addition of Tween80 had an enhanced effect on <i>G. lucidum</i> exopolysaccharide production in submerged fermentation. However, the mechanism of this effect remains unclear. In this study, we report on a high-quality assembly of <i>G. lucidum</i> strain yw-1-5 to lay the foundation for further transcriptome analysis. The genome sequence was 58.16 Mb and consisted of 58 scaffolds with an N50 of 4.78 Mb. A total of 13,957 protein-coding genes were annotated and Hi-C data mapped to 12 pseudo-chromosomes. Genes encoding glycosyltrJournal of fungi (Basel, Switzerland)Whole-Genome Sequencing and Transcriptome Analysis of <i>Ganoderma lucidum</i> Strain Yw-1-5 Provides New Insights into the Enhanced Effect of Tween80 on Exopolysaccharide Production.PMC96056142020GDASYL-202001030222018B0202050010Xie YCai MChen JWu QJiao CLiu YLi XZhang ZHu HWu TGao XChen SXiao CfalseWhole-Genome Sequencing and Transcriptome Analysis of <i>Ganoderma lucidum</i> Strain Yw-1-5 Provides New Insights into the Enhanced Effect of Tween80 on Exopolysaccharide Production.<i>Ganoderma lucidum</i> is an important medicinal mushroom widely cultured in Asian countries. Exopolysaccharides are bioactive compounds of <i>G. lucidum</i> with health benefits. Limited exopolysaccharide content hinders its extraction from <i>G. lucidum</i>. The addition of Tween80 had an enhanced effect on <i>G. lucidum</i> exopolysaccharide production in submerged fermentation. However, the mechanism of this effect remains unclear. In this study, we report on a high-quality assembly of <i>G. lucidum</i> strain yw-1-5 to lay the foundation for further transcriptome analysis. The genome sequence was 58.16 Mb and consisted of 58 scaffolds with an N50 of 4.78 Mb. A total of 13,957 protein-coding genes were annotated and Hi-C data mapped to 12 pseudo-chromosomes. Genes encoding glycosyltr2022-01-01T00:00:00Z2022 Oct2026-04-08T11:43:31.936Z2024-11-13T23:30:54.67ZS-EPMC96056143629464610.3390/jof8101081