{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9"],"submitter":["Shrestha Palikhe N"],"funding":["Western University","Canadian Allergy, Asthma and Immunology Foundation","Canadian Institutes of Health Research"],"pubmed_abstract":["<h4>Background</h4>CRTh2 is G protein coupled receptor for prostaglandin D2 (PGD)<sub>2</sub> expressed by immune cells that drive type 2 inflammation such as CD4<sup>+</sup> T cells (Th2), eosinophils and group 2 innate lymphoid cells (ILC2) as well as structural cells including smooth muscle and epithelium. CRTh2-expressing cells are increased in the blood and airways of asthmatics and severe asthma is characterized by increased activity of the PGD<sub>2</sub>-CRTh2 pathway. The <i>CRTh2</i> single nucleotide polymorphism (SNP) rs533116 G > A is associated with development of asthma and increased Th2 cell differentiation.<h4>Objective</h4>To examine whether CRTh2 rs533116G > A associates with asthma severity. Since severe asthma is more common in females than males, we performed a sex-stratified analysis.<h4>Methods</h4>Clinical data from asthmatics (<i>n</i> = 170) were obtained from clinic visits and chart review. Asthma severity was assessed according to ERS/ATS guidelines. Peripheral blood cells were characterized by flow cytometry and qRT-PCR. Genotyping was performed by TaqMan assay.<h4>Results</h4>Older females (≥45 years) homozygous for minor A allele of rs533116 were more likely to have severe asthma, lower FEV<sub>1</sub>, a higher prescribed dose of inhaled corticosteroid and more type 2 inflammation than females carrying GA or GG genotypes. Comparing females and males with the AA genotype also revealed that women had more type 2 inflammation.<h4>Conclusions and significance</h4>The polymorphism CRTh2 rs533116 G > A associates with severe asthma and type 2 inflammation in older females. This study reveals a gene-sex-aging interaction influencing the effect of CRTh2 on asthma severity."],"journal":["Frontiers in medicine"],"pagination":["970495"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9606418"],"repository":["biostudies-literature"],"pubmed_title":["The CRTh2 polymorphism rs533116 G > A associates with asthma severity in older females."],"pmcid":["PMC9606418"],"pubmed_authors":["Cameron L","Vliagoftis H","Kim RB","Shrestha Palikhe N","Mackenzie CA","Licskai C"],"additional_accession":[]},"is_claimable":false,"name":"The CRTh2 polymorphism rs533116 G > A associates with asthma severity in older females.","description":"<h4>Background</h4>CRTh2 is G protein coupled receptor for prostaglandin D2 (PGD)<sub>2</sub> expressed by immune cells that drive type 2 inflammation such as CD4<sup>+</sup> T cells (Th2), eosinophils and group 2 innate lymphoid cells (ILC2) as well as structural cells including smooth muscle and epithelium. CRTh2-expressing cells are increased in the blood and airways of asthmatics and severe asthma is characterized by increased activity of the PGD<sub>2</sub>-CRTh2 pathway. The <i>CRTh2</i> single nucleotide polymorphism (SNP) rs533116 G > A is associated with development of asthma and increased Th2 cell differentiation.<h4>Objective</h4>To examine whether CRTh2 rs533116G > A associates with asthma severity. Since severe asthma is more common in females than males, we performed a sex-stratified analysis.<h4>Methods</h4>Clinical data from asthmatics (<i>n</i> = 170) were obtained from clinic visits and chart review. Asthma severity was assessed according to ERS/ATS guidelines. Peripheral blood cells were characterized by flow cytometry and qRT-PCR. Genotyping was performed by TaqMan assay.<h4>Results</h4>Older females (≥45 years) homozygous for minor A allele of rs533116 were more likely to have severe asthma, lower FEV<sub>1</sub>, a higher prescribed dose of inhaled corticosteroid and more type 2 inflammation than females carrying GA or GG genotypes. Comparing females and males with the AA genotype also revealed that women had more type 2 inflammation.<h4>Conclusions and significance</h4>The polymorphism CRTh2 rs533116 G > A associates with severe asthma and type 2 inflammation in older females. This study reveals a gene-sex-aging interaction influencing the effect of CRTh2 on asthma severity.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-05-03T10:32:48.639Z","creation":"2025-05-29T16:10:59.711Z"},"accession":"S-EPMC9606418","cross_references":{"pubmed":["36314028"],"doi":["10.3389/fmed.2022.970495"]}}