{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["27(20)"],"submitter":["Milde R"],"pubmed_abstract":["Capsaicin, produced by diverse <i>Capsicum</i> species, is among the world's most popular spices and of considerable pharmaceutical relevance. Although the capsaicinoid biosynthetic pathway has been investigated for decades, several biosynthetic steps have remained partly hypothetical. Genetic evidence suggested that the decisive capsaicin synthase is encoded by the <i>Pun1</i> locus. Yet, the genetic evidence of the <i>Pun1</i> locus was never corroborated by functionally active capsaicin synthase that presumably catalyzes an amide bond formation between <i>trans</i> 8-methyl-6-nonenoyl-CoA derived from branched-chain amino acid biosynthesis and vanilloylamine derived from the phenylpropanoid pathway. In this report, we demonstrate the enzymatic activity of a recombinant capsaicin synthas"],"journal":["Molecules (Basel, Switzerland)"],"pagination":["6878"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9606859"],"repository":["biostudies-literature"],"pubmed_title":["Chemical Synthesis of <i>Trans</i> 8-Methyl-6-Nonenoyl-CoA and Functional Expression Unravel Capsaicin Synthase Activity Encoded by the <i>Pun1</i> Locus."],"pmcid":["PMC9606859"],"pubmed_authors":["Hoehenwarter W","Westermann B","Ditfe T","Schnabel A","Vogt T","Proksch C","Milde R"],"additional_accession":[]},"is_claimable":false,"name":"Chemical Synthesis of <i>Trans</i> 8-Methyl-6-Nonenoyl-CoA and Functional Expression Unravel Capsaicin Synthase Activity Encoded by the <i>Pun1</i> Locus.","description":"Capsaicin, produced by diverse <i>Capsicum</i> species, is among the world's most popular spices and of considerable pharmaceutical relevance. Although the capsaicinoid biosynthetic pathway has been investigated for decades, several biosynthetic steps have remained partly hypothetical. Genetic evidence suggested that the decisive capsaicin synthase is encoded by the <i>Pun1</i> locus. Yet, the genetic evidence of the <i>Pun1</i> locus was never corroborated by functionally active capsaicin synthase that presumably catalyzes an amide bond formation between <i>trans</i> 8-methyl-6-nonenoyl-CoA derived from branched-chain amino acid biosynthesis and vanilloylamine derived from the phenylpropanoid pathway. In this report, we demonstrate the enzymatic activity of a recombinant capsaicin synthas","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2026-04-08T11:49:47.67Z","creation":"2024-10-14T23:04:49.043Z"},"accession":"S-EPMC9606859","cross_references":{"pubmed":["36296471"],"doi":["10.3390/molecules27206878"]}}