biostudies-literatureRomeu MJFundação para a Ciência e TecnologiaEuropean Union's Horizon 2020 Research and Innovation ProgrammeH2020-MSCA-RISE 2016FCT/MCTES (PIDDAC)Foundation for Science and Technology (FCT) and the European Regional Development Fund (ERDF) in the framework of the program PT20204410https://www.ebi.ac.uk/biostudies/studies/S-EPMC9607013biostudies-literatureUnknown14(20)The development of environmentally friendly antifouling strategies for marine applications is of paramount importance, and the fabrication of innovative nanocomposite coatings is a promising approach. Moreover, since Optical Coherence Tomography (OCT) is a powerful imaging technique in biofilm science, the improvement of its analytical power is required to better evaluate the biofilm structure under different scenarios. In this study, the effect of carbon nanotube (CNT)-modified surfaces in cyanobacterial biofilm development was assessed over a long-term assay under controlled hydrodynamic conditions. Their impact on the cyanobacterial biofilm architecture was evaluated by novel parameters obtained from three-dimensional (3D) OCT analysis, such as the contour coefficient, total biofilm volPolymersThe Use of 3D Optical Coherence Tomography to Analyze the Architecture of Cyanobacterial Biofilms Formed on a Carbon Nanotube Composite.PMC9607013EMERTOX (grant 734748)Ph.D. grant (SFRH/BD/140080/2018), Scientific Employment Stimulus - Individual Call - [CEECIND/01700/2017] and the Scientific Employment Stimulus - In-stitutional Call - CEECINST/00049/2018project SurfSAFE (952471)LA/P/0045/2020 (ALiCE), UIDB/00511/2020 and UIDP/00511/2020 (LEPABE)Strategic Funding UIDB/04423/2020 and UIDP/04423/2020Mergulhao FJRomeu MJLima MJong EDVasconcelos VPereira MFRMorais JSjollema JSoares OSGPGomes LCfalseThe Use of 3D Optical Coherence Tomography to Analyze the Architecture of Cyanobacterial Biofilms Formed on a Carbon Nanotube Composite.The development of environmentally friendly antifouling strategies for marine applications is of paramount importance, and the fabrication of innovative nanocomposite coatings is a promising approach. Moreover, since Optical Coherence Tomography (OCT) is a powerful imaging technique in biofilm science, the improvement of its analytical power is required to better evaluate the biofilm structure under different scenarios. In this study, the effect of carbon nanotube (CNT)-modified surfaces in cyanobacterial biofilm development was assessed over a long-term assay under controlled hydrodynamic conditions. Their impact on the cyanobacterial biofilm architecture was evaluated by novel parameters obtained from three-dimensional (3D) OCT analysis, such as the contour coefficient, total biofilm vol2022-01-01T00:00:00Z2022 Oct2025-04-03T21:30:32.253Z2025-04-03T21:30:32.253ZS-EPMC96070133629798810.3390/polym14204410